The Nanomaterials Based On Cu And Their Application In Non-enzymatic Glucose Sensor

Diabetes is a kind of common disease, as the World Health Organization reported, about 350 million people suffer from diabetes. It is estimated that diabetes will become the seventhplagued disease in the world until 2030. Therefore the blood glucose detection is verysignificantly. Glucose sensor plays an important role in the area of diabeticmonitoring and control.The majority of commercially availableglucose sensors rely on immobilized enzyme for its good selectivity and high sensitivity. However, owing to the intrinsic nature ofenzymes, the activity of enzyme iseasily affected by temperature, humidity, p H, toxic chemicals, etc, this factors lead to the stability andreproducibility of the glucose biosensor become poor. As an alternative approach, non-enzymatic electrodes as glucose sensors are attracting much attentiondue to their advantages of considerably greater sensitivity and chemical stability.Recently, lots of nano materials have been researched and applied in glucose sensors, including Pt, Au, Ni and Nioxides, Cu and Cu oxides, alloys and composite materials prepared by carbon(carbon nanotube, graphene) with metal and metal oxides. Cu and Cu oxides attract attentions due to advantages such as better electrical conductivity, low-cost and good performance of electrical catalyze the oxidation of glucose. In this paper,electrodeposition was adopted for preparing self-surpporting electrode, in addition, graphene oxide served as surface-active agent for making hybrid materials which was applied for construct glucose sensors. Comparing with traditional nano materials,self-surpporting electrode and Cu/Graphene oxide/GCE electrode exhibits better stability, reproducibility and higher sensitivity. The main content of paper as following:First, aglucose biosensor was fabricated by self-surpporting stalagmite-like Cu electrode, self-surpporting stalagmite-like Cu electrode with evenly arranged array structure was prepared through potentiostatic electrodeposition. The morphology of the prepared self-surpporting stalagmite-like Cu electrode was characterized by scanning electron microscopy(SEM), x-ray diffraction(XRD). The amperometric detection of glucose was carried out at the potential of 0.55 V, with a wide linear range from 1.0μM to 3.0m M(R2=0.9947), a low detection limit down to 0.33μM(signal/noise = 3), and a higher sensitivity of1370μA?m M-1?cm-2.Inshort,the self-surpportingstalagmite-like Cu shows advantage in glucose detection for the good stability,excellent reproducibility,high selectivity and short response time(<3s). In addition, the practical application was investigated in this paper, the resultsapproximate to the values tested by hospital, it shows thatself-surpportingstalagmite-like Cu exhibited good properties in practical application.In consequence,the abovefavorable properties indicate that the as-prepared self-surpporting stalagmite-like Cu electrode has great potential in practical applications as non-enzymatic glucose sensor.Second, solvothermal pathway was developed to prepare composite material of Cu and graphene oxide in this paper, Cu/Graphene oxide/GCE electrode made by the method of coating. It can be seen from the result of amperometric test that Cu/Graphene oxide electrode exhibits remarkableelectrocatalyticactivity such as good selectivity, excellent reproducibility and outstanding stability towards glucose oxidation in both alkaline medium and human serum. The sensitivity of the glucose detection is 1120mA·m M·cm-2, with a widerlinear range from 3mM to 8.5m M(R2=0.99655), a low detection limit1.0μM(signal/noise=3).In addition, the practical applicationof Cu/Graphene oxide electrode was also tested in this paper,the results approximate to the values obtained by hospital. All these results indicate thisnovel nanostructured composite material is a promising candidate for non-enzymatic glucose sensors.