Analysis Of Fungicide And Antiseptic In Food By Liquid Chromatography

It is of great significance to ensure food safety. With the application of pesticides in agriculture and unreasonable use of food additives, food safety problems occur constantly.First of all, in this paper, the chromatographic analysis research progress of fungicide and antisepsis in food at home and abroad in recent years were reviewed. In the second chapter,floated organic drop microextraction(FDME)-liquid chromatography was established for determinating in cider wine. The fungicides were separated by a reversed-phase Diamonsil C18 column. The mobile phase was the mixture of methanol, acetonitrile and water. The detection wavelength was 225 nm. N-dodecyl alcohol was used as extracting agent. The effects of extractant, p H, salt addition were investigated. Under the optimal conditions, the result show that baytan, 2,4,6-trichlorophenol and prochloraz exhibited good linearity with the correlation coefficients above 0.9992. The method detection limits were 0.4, 0.8 and 4.0ng/m L, respectively, the average recoveries were 101.1, 102.1 and 106.3%, respectively. The relative standard deviations(RSD) were less than 3.8%.In the third chapter, with anhydrous sodium sulfate as dispersant, thiabendazole, baytan, folpet and prochloraz in raisins were extracted by Solid phase dispersion method, the C18 solid phase extraction column was used for purification. Four kinds of fungicide and antiseptic were separated by reversed-phase high performance liquid chromatography with C18 column. The mobile phase was the mixture of acetonitrile, methanol, water. The linearity was good in the range of 0.01~200.00 μg/m L, with correlation coefficients were greater than 0.9993. The method detection limits were2.00~20.00 ng/g, the average recovery were 70.5~97.3%. The relative standard deviations(RSD) were 1.39~3.18%. In the last chapter, 2-naphthol, p-phenylphenol, diphenyl ether,prochloraz in the kumquat tea were determined by HPLC. Acetonitrile was extractant,subzero-temperature frozen extraction and florisil solid phase column purification were applied to the sample treatment. The target compounds were separated by C18 column and detected by variable wavelength UV. The mobile phase was mixture of methanol and water.There was a good linearity in the range of measuring amount, with correlation coefficientsabove 0.9991. The method detection limits were 2.0~50.0 μg/kg. The average recovery were more than 78.0% except diphenyl ether, The relative standard deviations(RSD) were less than 4.1%.