Improvement Of Magnetic Dispersive Solid-phase Extraction And Interferon Purification Technology

In recent years, the quantity and quality of drugs in our country have significantly improved because of the development of new pharmaceutical techniques and technology. One of the key techniques in pharmaceutical industry is separation and purification, which has been one of the standards to measures the level of modern pharmaceutical industry. The performance of separation and purification techniques, that directly affects the purity, yield, safety, and curative effect of drugs, has been paid more and more attention by many researchers. The development of science and technology promotes the supplement and optimization of separation and purification techniques and many techniques have emerged, such as extraction, membrane separation, ion exchange, chromatographic separation and so on. The work of separation and purification techniques mainly includes: 1. To choose proper separation methods to extract pharmaceutical ingredients; 2. To remove ineffective and harmful components and retain effective components and parts with purification and refinement techniques.This paper summarized the research of sample separation and purification techniques, and mainly focused on the following items:1. The development of separation and purification techniques in pharmaceutical industry was summarized. Especially, sample preparation and protein separation techniques, and the progress and outlook of their applications in pharmaceutical production were mainly introduced.2. Magnetic graphene/carbon nanotubes hybrid material was prepared by a modified Hummers method combining with sedimentary doping technique, and used as adsorbent and developed magnetic dispersive solid-phase extraction to extract and separate oxytetracycline in pharmaceutical wastewater. Parameters that affect extraction efficiency were investigated and optimized.3. The separation and purification process of recombinant human interferon α2b was improved and the quality of α2b original fluid was promoted to increase the output and reduce the cost. Variety parameters in experiment processes were verified to ensure the quality standard of original fluid.