| Pen/Ia frutescens (1.) Britt is a kind of Labiate plant which has been used as food and medicine from ancient times. It has important physiological and health protective value that makes it significant in food industry and medicine field. Perilla oil contains a large quantity of fatty acid that makes it has many physiological and sanitarian functions, such as decreasing blood lipid, decreasing blood pressure, antianaphylaxis, antisenescence and anticancer. There is Baicalein in penilla frutescen.s stems and leaves, which has many functions, such as adjusting blood fat, antioxidance and antibacteria. JnilIa frutescens essential oil contains some active elements with antimicrobial activity, which are L-perillaldehyde, L-limonene cinene, P -pinene, a -pinene, linalool etc..In order to study the antimicrobial activity of peril/a frutescens systematically, its stems, leaves and seeds were taken as materials. With Stilled water, alcohol and petroleum ether as solvents, the effects of drying methods and solvents on extraction ration and antimicrobial activity were reviewed, the effects on extraction ration of ratio between solvents and material, temperature and time were investigated by means of single factor and orthogonal experiment and the optimum extraction condition was determined, furthermore, the antimicrobial activity of different organs and the same organ gathered in different growing periods were compared. With Eschericbia coil, Bacillus subtiiis, Staphylococcus arueus and some molds and yeast as experimented microbes, the characteristics of antimicrobial activity were studied by means of filter paper method and plate dilution method. Extract was separatedwith Thin Layer Cbomatogfaphy and applied In the prese。ations of stewed meat,dried beau curdandplckled celery．he results Indicated:l．Stilled water,alcohol and petroleum ether extfacts had bacteno删Is to germs,moulds and yeast．he Inhlbltlon sequence to experimented microbes ofstllled waterand alcohol extract was:to germs＞to yeast＞to molds．But Stilled water extract hadthe best a－ntrmlcroblaleneet on germs,tvlth the diameter of nimttzoblal un卜Escherichla coil beyond 10 tnm ndndalcohol extract h劝 better m口bltlonto yeast aMmoulds than that ofstllled water．刀怕盯山mcroblal activity ofpetroleum ether wasinfenor to the above two．2．Drying Inetllods hM s侣mfie'effect on e航action ration and antlmlcroblalunctlon and the difference was siwficat卜0．of)．The raw matenal dried byInlcrowave had the highest extraction ration ith 7．33％,the second higher of7．03％appear叨 In山aUrlcd加 hotmr,山e rrd吨her of6．93％ppeard m山a巾ned叶room temperature and the smallestwas m that dued by hot ir after scalded,w山＊was ody 6．50％．he山ameter ofantinucroblal nng to Eschencma coil ofrawmaterial吐n叨 by microwave was 8．7nun,wmchwasthe most In fourtrea加ents,thesecondo18．3mm was Inthat吐nedby roomtemperarre,the山吐d ofs．lrnmappeared In that dued hp hot air andthe last one was Inthat dued by hotair afterscalded,which was 7．4nun．3．The eftCct on extlactlon ration ofmo between solvents and raw material andtemperature were greaterthanthatoftime．Alcohol as solvent,the optimumexwctlon condition was:ratio beMen solvents and raw material:12:l,temperature:80"c,time:l．sh,At this condition,the extraction ration was 7．40％．4．The antimlcroblal。tlvityof different Perilla frutescens organs and hat of thesame organ growed In dl地rent penods were dl地rent．he antimlcroblal activity ofstems ppd！eaves wasbeneroanouofseeds．aems and阶yes g毗ered Inmesecond part ofjuly had better antlmlcroblal activity than others．5．Perilla fFutescens alcohol extract had ide antlmlcroblal spectnim．Its minimall山Mbltory concen订atlon to germ 'vas 0．625％and that to moulds and yeast was1．二5 0／O0／O． 46.Peril/afrutescens alcohol extract had wide pH active range. When base pH was 3 to 7, it had ob... |
PDF Full Text Request