Drug Interactions Of Biological Macromolecules And Its Analysis Determination

In 1871, Friedrich Miescher, a scientist of Switzerland, firstly separated the complex of nucleic acid and protein named 搉uclein?from the pus in the bandage. His dissertation pioneered the study of nucleic acid and protein for the future. More than one century passed, now we have cognized that they are important chemical substances in our life. Nucleic acid and protein are the main targets of all medicines in organism. Their binding formations and sites influence the pHysiological and pHysi-chemical characters of biomolecule. Because it is helpful to design new medicine, study healing principle and toxicity of medicine etc., it is of special value to study the interaction of biomolecule and druggery. In this paper, we study the interaction of druggery and biomolecule by molecule spectroscopy (fluorescence and UV-Vis spectroscopy), spectroelectrochemistry methods. There are four chapters. First, we studied the interaction of Human Serum Albumin, Bovine Serum Albumin and. drugs such as antibacterial Ketorolac, Feprazone, antibiotic Cefoperazone. Applying fluorescence quenching method and energy transfer technique, we have studied the quenching mechanism of protein and drugs. The binding sites and binding constants were obtained. According to the thermodynamic parameters, the main sorts of binding force can be known. Second, the interaction mechanisms of calf-thymus deoxyribonucleic acid (ct-DNA) and Polymyxin B, Berberine Hydrochloridum with EB as a fluorescence probe were discussed. We studied their fluorescence spectrum, UV-Vis spectrum, fluorescence polarization, melting curve etc. 3 The results show that Polymyxin B interacts with ct-DNA by intercalation and eletrostatic gravity. Berberine can intercalate between the basic pairs of the double-helical structure of ct-DNA, so that its fluorescence increases markedly. The quantity of Berberine can be measured by using the linear relationship between the concentration of Berberine and the fluorescence intensity with the existent of amount of ct-DNA. Third, a new synchronous fluorometric method for the simultaneous determination of Aloe-emodin (AE) and Rhein (RH) is proposed. This method is applied to the simultaneous determination of AE and RH in simulated mixture and the amount determination of AE in Cassitora L. At the same time, we establish a fluorescence method to analysis Aloe- emodin. This developed method is successfully applied to the determination of Aloe-emodin in samples of Cassi tora L, Serum and Urine. The results are satisfied. Fourth, the study of spectroelectrochemistry is in progress. In this paper, a simple convenient self-made thin-layer cell is designed. However, there are a series of unperfect problems in this cell. It needs further betterment in the future work.