Quinolones And Nucleic Acids, Protein Interaction And Its Analytical Determination

Nucleic acid is the carrier of genetic information. It is also the determination factor for species' continuation and evolution. Protein has varieties of life and is the direct expresser of biological shape and properties. It is very important for researchers to expound the secrets of life to develop new functionalized medicine to surmount many difficult diseases and promote the development information science. What we should do is to further penetrate the interaction mechanism between small molecular and nucleic acid or protein and to develop rapid and c convince assay for nucleic acid or protein, This project is the forward position and hot point in bio-analytical chemical research.Quinolones' drug is the extensive antibacterical drug, which has developed rapidly and utilized widely in clinical. It has many advantage ,such as, the strong energy of antibiosis, the extensive antibiosis, only few bacterial strain endured drug ,little side effect , not taken orally but injected in vein ,and so on. The research in basic science and in clinical on the drug is a hot topic of conversation. And the research on analytical determination is the important aspect.In this paper, there are two aims with the two above. We study the interaction of quinolones' drug and biomolecule by means of fluorescence and UV-Vis spectroscopy. There are three chapters.First, the third of quinolones' drug has the transition of molecule energy with the rare-earth metal ion Tb3+ ,but DNA can strengthen the fluorescenceintensity of system. So it can test DNA as fluorescence probe. In this chapter, we study the fluorescence intensity effect and the influence factor of the nucleic acid with the system of norfloxacin or eroxacin-Tb3+, we investigate the interaction mechanism and advance the model interaction of the mixture and nucleic acid, and build the optional determination of DNA.Second, we studied the information of drug and protein by fluorescence spectroscopy. Such Human Serum Albumin, Bovine Serum Albumin and lysozyme as the main and such the enoxacin which is the third generation quinolones' drug as the object. We have studied the quenching mechanism of protein and drugs,. The binding sites and binding constants were obtained. According to the thermodynamic parameters, the main sorts binding force can be known.Third, quinolones' drug can make the effect with the rare-earth metal ion Tb3+ and strengthen the characteristic fluorescence of Tb3+. We can make the determination of Quinolones' drug with the character. With the condition of the existence of DNA, it can strengthen the fluorescence intensity of the system above. We have further improved the method of fluorescence determination of Quinolones' drug on this base, and applied successfully in the determination of norfloxacin and enoxacin.