| Capillary Electrophoresis (CE) has become one of the fastest technique in recent years because of its high efficiency, rapidness, good sensitivity and simple instrument, which are necessary for instrumental analyse. In the present paper, a series of investigation has been developed in principle and application on capillary zone electrophoresis with amperometric detection and the third is capillary zone electrophoresis with amperometric detection for composition analysis of Laminarin.Chapter 1 ReviewThe first section has reviewed the history, the current situation on High Performance Capillary Electrophoresis (HPCE). Second one has introduced the fundamental theory of HPCE and its characters, detection technique and sample injection, individually. Third one is about the application of HPCE, which includes analyses of inorganic and organic small molecules, food, Chinese herb, chiral molecules, medicine, environmental, biomacromolecules etc. The last one is about the purpose and meanings of this dissertation.Chapter 2 Effect of diluting agent on sensitivity in capillary electrophoresis with amperometric detectionBesides the running buffer, pH of buffer, separation voltage and sampling time, the diluting agent was studied in this paper as one of the factors influencing the sensitivity in capillary electrophoresis (CE) with amperometric detection (AD). Clonidine hydrochloride, hydrochlorothiazide and rutin, which are positive charged, neutral and negatively charged respectively in aqueous solutions, could be perfectlyseparated by CE with 20mmol-L-1Na2B4O7- 40mmol-L-1 NaH2PO4 as running buffer and detected by measuring their current responses with AD. Before CE running, water, methanol, formamide, running buffer, hydrochloric acid and sodium hydroxide were applied as diluting agents in this experiment to investigate their effects on the sensitivity of CE-AD. The results showed that the current responses of these three analytes were great affected in different appearance when the running buffer was constant but different diluting agents were used. This method was applied to simultaneously determine the active ingredients in one Chinese compound hypotensor named Zhen Ju Jiang Ya Pian, in which the contents of clonidine hydrochloride, hydrochlorothiazide and rutin is very different as 0.03mg : 5mg : 20mg per tablet, and satisfactory results were obtained by adjusting their sensitivity by selecting the suitable diluting agent. Chapter 3 Study on determination of hydroxyl radical by capillary zoneelectrophoresis with amperometric detectionHydroxyl radical (OH ) is the most aggressive one among these reactive oxygen radicals and cause cell injury when they are generated in excess or the cellular antioxidant defense is impaired. However, OH is very difficult to be detected for its very reactivity and very short half-time In this paper, the simple and high sensitive capillary zone electrophoresis with amperometric detection (CZE-AD) was introduced to determine OH- produced by Fenton system by determining its reaction products with salicylic acid (SAL): 2,3-dihydroxybenzoic acid (2,3-DHBA) and 2,5-dihydroxybenzoic acid (2,5-DHBA). Under the optimum conditions, SAL, 2,3-DHBA and 2,5-DHBA could be perfectly separated within 15 min. Their detection limits were as low as 2 10-8 mol l-1, which were much better than CE- UV method. The method was also applied to study the scavenging activity of angelicapolysaccharide. The experimental results showed that the introduced CZE-AD method was very sensitive and practical in both the determination of free OH and evaluation of the activities of OH scavengers. Chapter 4 Capillary Zone Electrophoresis with Amperometric Detection forComposition Analysis of LaminarinThe composition of laminarin was firstly determined by analyzing its hydrolysis monosaccharides with capillary zone electrophoresis - amperometric detection (CZE-AD). Under the selected optimum conditions, fucose, galactose, glucose, mannose and xylose, which are hydrolysis products of laminarin, could... |
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