Cassia Seed Functional Components - Anthraquinone, Extraction And Analysis Methods

This thesis has studied the extraction new methods of functional Components of anthraquinone and carbohydrates and sugars of Cassia obtusifolia L. ,and have put forward new methods of determination of anthraquinone. The methods have been applied to assay and extraction the samples with satisfactionary results. This thesis is devided into seven parts:Part I : Review. A review of the extraction and determination methods of anthraquinone and carbohydrates and sugars in Cassia obtusifolia L.is given.Part II: Study on extracting component carbohydrates and sugars in Cassia obtusifolia L. by ultrasound. The conditions for the extraction of carbohydrates and sugars by ultrasound were optimized by the design of orthogonal experiments. Compared with that of the heat backward flow usually used in medicinal industry, this method has a higher extracting velocity and rate. Results indicated a better effect was time of extraction of heating is 30 minutes,and times of extraction are 2 and the proportion of extraction is 1:40. Under this conditions, a extracting rate of 10.60% can be reached This method shorts time of extraction ,saves energy and avoids to damage structure of carbohydrates and sugars.Part III: Studies on process for extraction of anthraquinone from Cassia obtusifolia L. by orthogonal experiments. Solvent of extraction of anthraquinone was chooesed as guide by the content of total anthraquinone present in the extraction .Conditions for the extraction were studied by the design of orthogonal experiments. Results indicated a better effect was 80% ethanal as solvent.Time of extraction of heating is 2 hours,and times of extraction are 3 and the proportion of extraction is 1:10.Part IV: Study on extraction of anthraquinone from Cassia obtusifolia L.by ultrasound. The method of extraction the anthraquinone from Cassia obtusifolia L. by ultrasound is studied. Compared with that of the heat backward flow usually used in medicinal industry, this method has a higher extracting velocity and rate. Because ultrasonic extraction need not heat, the effect of heating to theanthraquinone can be avoided.Part V: Determination of anthraquinone by flow injection chemilu-minescence in Cassia obtusifolia L.. A reverse flow injection chemiluminescencemethod for the determination of anthraquinone was firstly described. It was found that the CL intensity from the luminol-ClO- system could be reduced in the presence of anthraquinone. The CL emission was inversely correlated with the anthraquinone concentration in the range from 0.5 to 100ug/mL, the detection limit was 0.05ug/mL and the relative standard deviation was 1.6%, a sampling frequency of 150 h-1 was attained .The recovery was 103.1-105.2%.When the method is applied to determined anthraquinone, it can obtain good result.Part VI: Kinetic Spectrofluorimetric Detrminatiion of Anthraquinon in Cassia obtusifolia L.. A simple and sensitive kinetic fluorimetric method is reported for the determination of anthraquinon in alkaline aqueous solution. The detection limit for anthraquinon is 0.2ug/mL. The liner range of the determination is 0.4~50ug/mL with the recovery was 104-106%. It can be successfully applied to assay of anthraquinone .Part VII: Determination of metals in Cassia obtusifolia L. by atomic abosorption spectrometry. There are ample trace elements in Cassia obtusifolia L. It is very important to determination them by the angle of pharmacology and nutritional science. A simple, rapid and accurate method for the assay of trace elements in Cassia obtusifolia L. has been developed by atomic abosorption spectrometry. This furnishs means to appraise quality of crude drugs and develops health foods. The relative standard deviation was 1.3-3.5% and recorvy was 91.2-102.5%.