White Rot Fungi On Biofilm Treatment Of Dye Wastewater

The study on decolorization of Phanerochaete chrysosporium & Trametes versicolor to dye wastewater and degradation of biofilm reactor using Trametes versicolor to dye waste-water was carried out in this dissertation .The basic parameters of decolorization to China green and Safranine T using two kinds of white rot fungus in shallow static culture were confirmed by the experiments, and decolorization of them to 7 kinds of dyes with different structure was observed. The result showed that decolorization of Phanerochaete chrysosporium to heterocyclic dye was not efficient, while Trametes versicolor efficiently degraded dyes from all groups. Trametes versicolor were selected and used for degradation of one kind of dye (China green) & mixed ones (Methyl orange, China green &Safranine T mixed at the same concentration and volume) by using the aerobic biofilm contact oxidation, adopting intermittent operation types.The result showed that the maximum of critical influent substrate concentration of China green could be over 200ppm after short-term inducement, and the rate of decolorization attained over 90%(HRT=4h).To mixed dye with high concentration (90ppm respectively), the rate could attain 94.7% in the same HRT. Decolorization was proved to be independent of light by means of experiments. The theoretical critical influent substrate concentration of China green was ascertain applying substrate removal dynamics model to the reactor, and the value tallies with the experiments' one.The GC-MS mensuration to China green showed that its structure has changed after decolorization, and the TOC mensuration to mixed dye showed that Trametes versicolor could remove organic pollution of dyestuff substrate efficiently in short time. In the experiments, the following phenomenon was observed, two kinds of fungi will degenerate after their propagative generation number exceed 6.Some rejuvenation methods to the strains were recommended .