Nicotine Ingredient In Tobacco Research

This　study　was　aimed　at　nicotine　contents　in　the　same　kind　of　tobacco　by　different　kinds　method　,specially　at　the　study　is　change　of　nicotine　contents　in　tobacco　after　addition　of　Alone　　sample　by　　HPLC　process.　Nicotine　is　primary　component　in　tobacco　by　Utilizing　GC-MS　and　nicotine　contents　are　6.50　mg/g　by　GC　method　with　the　recovery　and　coefficient　of　variation　are　99.87%　and　1.26%.The　optimum　conditions　for　extraction　was　proved　practicable　by　condition　filtration　and　orthogonal　design　with　　supersonic　wave　method　.The　results　showed　that　the　optimum　conditions　for　extraction　to　ultrasonic　method　is　:　methanol　(φ=40,20ml),and　15℃　for　30　min　,this　paper　has　determined　nicotine　contents　that　is　23.50mg/g　at　absorption　peak　(λ=262nm)　by　HPLC　with　an　eluent　consisting　of　methanol　–potass　Phosphate　alleciate　(pH=3)　with　the　recovery　and　coefficient　of　variation　are　101.52%　and　0.91%.　The　results　of　HPLC　method　are　true　and　reliable　and　sensitive　and　the　method　save　sample.　Adding　different　breeds　,different　places　of　Alone　in　tobacco　,in　the　light　of　ultrasonic　method　dealing　with　samples　,nicotine　content　was　determined　　by　　assured　HPLC　method　,　The　results　showed　that　Alone　　can　　depress　nicotine　contents　in　tobacco　,all　places　are　good　to　depressing　nicotine　in　tobacoo,　its　peel　of　leaf　and　dry　power　are　prominent　.Leaf　and　peel　of　leaf　of　A.arborescens　are　notable.　To　　sum　　　up,Alone　vera　　L.　is　more　than　　A.arborescens.　　　　　This　paper　systematically　makes　a　series　of　experiments　on　the　nicotine　in　tobacco,analyzing　the　received　results　more　completely　and　scientific　methods　to　the　research　on　nicotine　and　the　rational　development　of　Alone　.