The Pittosporum Skin Anti-tumor Effective Parts Of The Preparation Process And The Quality Of Research

This dissertation included three parts: summary of related literatures; study on the chemical constituents of Haitong-pi from Cheng du, establishment the quantitative determination of hedragenin-3-O-α--L-rhamnopyranosyl-(1â†'2)-α-L-Arabinopyran- oside (kalopanax saponin A) and total saponins, HPLC fingerprint of effective parts from Haitong-pi by High Performance Liquid Chromatography (HPLC) analysis and discussion of experiment.On basis of its spectral data, it was elucidated as hedragenin-3-O-α-L- rhamnopyranosyl-(1â†'2)-α-L-arabinopyranoside (kalopanax saponin A). chromato- graphic fingerprint of Haitong-pi by High Performance Liquid Chromatography (HPLC).The documents about the present research situation in Chinese medical haitong-pi were summed up and analyzed. A review on the research progress of Haitong-pi in chemical constituents and pharmacological activities was presented.Experiments about Haitong-pi.The observation of the extracted term of the effective fractions of Haitong-pi Compound with the amount of the total saponins as evaluation index give the result that the best extracted term is that the drug is extracted by Q1% alcohol for three times, 6 times and two hour.Observing the absorption fraction with the amount of the total saponins of Haitong-pi Prescription as the evaluation index. On four kinds of macro absorption resins, we reach the conclusion that the A resin has the best absorptive ability on the separation and enrichment of the effective fractions from Haitong-pi Prescription and the best technology term is that the ratio of the amount of sample and volume of resins is 1:3.5, the extraction substance with 20mg/ml density passes the resin column that D/L is 1:5 with the 1ml/min absorbed speed, washed out withQ3% alcohol for 6 times of resin volume with 3ml/min eluted speed. This method is simple and can be put into practice.The chromatographic fingerprint of effective fractions of Haitong-pi are...