Study On The Preparation Technology And The HPLC Fingerprint Chromatograph Of Yikangjucha

Yikangjucha is a kind of health beverage, which is made of the extracts from FmctusSchisandrae Chinensis, Radix Astragali, Fructus Lycii, Fructus Crataegi and Flos Chrysanthemi.The source, efficacy, chemical constituents and pharmacological effects of the five Chinesetraditional medicines were summarized.The optimum extraction process of Fructus Schisandrae Chinensis was selected by theorthogonal experimental design with the content of schisandrin as an index. The optimum extractionprocess of Radix Astragali, Fructus Lycii, Fructus Crataegi and Flos Chrysanthemi was investigatedby the orthogonal experimental design with the content of astragalosideⅣas an index. The refiningtechnology of the effective components of extract was optimized by an orthogonal design using thecontent of schisandrin, astragalosideⅣ, polysaccharide and the clarity of solution as indices. Thepreparation technology of Yikangjucha was established.HPLC fingerprint of Yikangjucha was achieved after chromatographic parameters had beenstudied. Total of 29 shared peaks were determined as characters of the fingerprint. Their individualcharacteristic peaks of the five medicinal plants (Fructus Schisandrae Chinensis, Radix Astragali,Fructus Lycii, Fructus Crataegi and Flos Chrysanthemi) were also identified. The results of themethodological studies indicated that this HPLC fingerprint is of good precision and reproducibilityas well as being highly stable, conforming to the establishment principle of TCM fingerprint. Thefingerprint can fully be used for the qualitation evaluation of Yikangjucha.Reverse-phase HPLC methods were developed for the determination of schisandrin andastragalosideⅣin both the raw materials and Yikangjucha. Stability of Yikangjucha wasinvestigated based on the technical regulations of the approval for health products required by theMinistry of Public Health in China. Validity duration of Yikangjucha is tentatively set on as 1 year.The antioxidant effects of the extracts from Yikangjucha and each medical material in vitro were studied. Results indicate that the extracts from Yikangjucha and each medical materialexhibited a significant potency inhibiting spontaneously lipid peroxidation in rat liver. The hydroxylfree radical induced by Fenton reaction could be scavenged effectively.