Sepiolite Immobilized Decolourizers The Dyestuff Wastewater,

An experimental system was set up for immobilization by sepiolite, it had many features, such as excellent stability, low cost, long life, small mass transfer resistance, recyclable and so on. The paper studied on the structure and chemic features of sepiolite at different heat-treated temperatures, and the basic features in de-ionized water and inorganic culture medium. From structure and nutrition points of view, feasibility and every influence factor were discussed. Also, the immobilized decolorizing strain was supplied to dealing with waste water containing acid red B, the influence of activation temperature, circumstances temperature, pH value, the amount of carrier and microbe, the rate of shaker, dye concentration and salt degree on the strain decolorization were investigated, and the degradation of different structure dye was also studied. The process and principle of degradation were analyzed through UV — Vis spectrum.It was showed that sepiolite was able to be an optimum immobilization carrier. Not only the amount of microorganism in the system increased, but also the applicability of bacteria in different circumstances enhanced. The results also indicated that sepoilte heat-treated in air at 400℃ was the best immobilized carrier of the strain, and the suitable conditions for immobilized cells were determined as follows: temperature 33℃, pH7～9, 120r/min for the rate of shaker, 72h for combining. The optimum conditions for the dye decolorization of immobilized strain were temperature 33℃, pH4～9, the carrier amount of 10g/L, the microbe amount of 2% and the shaker speed of 120 r/min, the highest decolorizing rate could reach 94%, and the influence of salt degree was small. The decolorization of mono-azo dyes was easier than disazo dyes. UV — Vis spectra showed that the conjugate structure and aromatic part of acid red B were destroyed after degradation.