Biologically Functional Structure Of The Mixed Complexes Of DNA Binding

According to the theoretic significance and the applications of the mixed ligands complexes, this text mainly completed the following work:1. Synthesis. In mixed solution of aqua-ethanol, three novel transition metal ion (Ni²⁺ , Zn²⁺ , Cd²⁺ ) complexes with N-(2-propionic acid)-salicyloyl hydrazone (C₁₀H₁₀N₂O₄, H₃L) and imidazole (C₃H₄N₂, Im) have been synthesized, and their single crystals were obtained. Eleven rare earth complexes containing 2, 6-pyridine dicarboxylic acid (H2PDA) and H3L have been synthesized. Two of them have been determined by X-ray single diffraction. And nine mixed ligand complexes RE₂(HL)(L)(inic)·4H₂O (RE = La,Ce,Pr,Nd,Sm,Eu,Gd,Dy,Er,Tb)also have been obtained.2. Characterization. The structures and photo-physical properties of these complexes obtainted above have been characterized by elemental analyses, IR spectrum, UV-Vis spectrum, Excitation spectrum, X-ray powder diffraction, X-ray single diffraction and TG-DTG, the composing of the complexes are confirmed. Furthermore, the crystal structures of some complexes have been determined.3. Property measurement. The bioactivity against coliform of the complexes RE₂(HL)(L)(inic)·4H₂O(RE = La,Pr,Nd,Sm,Eu,Gd)and Zn(HL)(Im)·H₂O are tested by Photoelectric Turbidimetry method. The complexes Nd₂(HL)(L) (inic)·4H₂O, Ni(HL)(Im)₂H₂O and Zn(HL)(Im)H₂O are selected to test for antimicrobial activity against Puccinia striiformis Westendorp by spore sprout method. DNA binding mechanisms of the complexes are observed by UV-Vis spectrum and fluorescence spectrum analysis.