| Solid substrate-room temperature phosphorescence (SS-RTP) has advantages of high sensitivity, wide linear range, less sample usage, rapid and simple. In recent years, this analytical method has been widely used in the life sciences, environmental sciences, clinical medicine, law seized, industrial hygiene and the energy field, especially in the analysis of pesticide residues, polycyclic aromatic hydrocarbons (PAHs), nitrogen heterocyclic compounds (NHCs) and medicines in biological or environmental samples. However, the preparation of the solid substrate which can induce strong RTP and have low background and the application of synchronous scanning technology in SS-RTP are still important study topics, in this thesis:Chapter one: the mechanism, application and prospect of SS-RTP were introduced in detail. The luminescence mechanism and impact factors of RTP, the application ofβ-cyclodextrin (β-CD) in RTP, the principle of solid phase extraction technology and synchronous scanning technology were described completely.Chapter two: the experimental method of Pullulan substrate was developed. Experimental conditions of the preparation of Pullulan substrate was optimized, including the amount ofβ-CD and 2-bromoethanol, the stir time and drier conditions, and RTP behavior of 15 organics on the Pullulan substrate were studied. The results indicated that the intensities of RTP of several PAHs and NHCs were selectively intensified on the Pullulan substrate, and the sensitivities of determination of them were enhanced.Chapter three: an organic polymeric monolithic substrate was prepared by situ polymerization and modified by a reaction withβ-CD. Theβ-CD modified substrate can be used as solid substrate and solid phase extraction (SPE) membrane for solid phase extraction-room temperature phosphorescence (SPE-RTP). The synthesis conditions, physical properties and chemical properties ofβ-CD modified substrate were studied, and theβ-CD modified substrate shows low RTP background, high selectivity and extraction ability. After SPE, the limits of detection (LODs) of organics with two or three rings were decreased to sub ug/mL level. The developed method was applied to the determination of fluorene and phenanthrene in water samples and biosamples, and the recoveries for fluorene and phenanthrene in samples mentioned above were 76.7%~101.7% and 83.3%~92.0% respectively. For 3 measurements, RSDs were below 8.06%.Chapter four: carbazole was determined by synchronous scanning with solid substrates-room temperature phosphorescence. Some experimental conditions were examined, including drying time, heavy atom and the constant wavelength interval. This method was applied to the determination of carbazole in environmental water samples, and the limit of detection (LOD) was 6.30ng·mL-1, the RSD was 3.67% and the recovery was 94%~109%.Chapter five: a method for the determination ofα-Naphthylacetic Acid (α-NAA) and 6-Benzylaminopurine (BAP) simultaneously by synchronous - derivative solid substrate-room temperature phosphorimetry was described. With pH9.5, 1.5mol·L-1KI-CH3COOLi as heavy atom salt, andΔλ=180nm, the overlapped phosphorescence spectrums ofα-NAA and BAP were well separated, the two synchronous derivation phosphorescence peaks are located at 305nm and 257nm. Forα-NAA and BAP, the absolute detection limit is 14.88ng·spot-1 and 6.75ng·spot-1 respectively. The method was applied to the determination ofα-NAA and BAP in vegetable samples, and the recoveries were 95.0%~102% and 92.7%~97.7%. |
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