Beta-cyclodextrin Filter Paper In The Solid-phase Extraction And Solid Substrate Room Temperature Phosphorescence Analysis

Polycyclic aromatic hydrocarbons (PAHs) and nitrogen heterocyclic compounds (NHCs) are of carcinogenesis and deformity and unfavorable to human health. Their chemical and physical stability makes them ubiquitous in the environment, particularly in the aquatic ecosystem, so oceans, rivers, and lakes act as ultimate PAHs and NHCs environment reservoirs. Due to their hydrophobic and lipophilic character, PAHs and NHCs tend to accumulate in sediments and biota and thus their concentrations in water samples are extremely low. The partitioning process into sediment and biota results in low water concentration levels. Therefore, high sensitive analysis techniques combine with the preconcentration process for the determination of PAHs and NHCs in the water samples are often required. The solid phase extraction - room temperature phosphorescence(SPE-RTP) is an effective method for the determination of PAHs, PCBs and so on. So our works focus on the further development and perfection of SPE-RTP.In chapter 1, the mechanism, application and prospect of SPE-RTP were introduced in detail. The luminescence mechanism of RTP, impact factor and the principle of SPE, instrument and the application and development of SPE-RTP was described completely.In chapter 2, the experimental method of p-cyclodextrin(p-CD) modified chromatography filter paper was developed. Experimental conditions of beta-cyclodextrin bonded chromatography filter paper was optimized, includingsynthetical mode, the dosage of epichorohydrin, activation time and the kind of filter paper, and RTP behavior of 16 organics on the bonded filter paper were studied. The result indicated that the intensities of RTP of several PAHs and NHCs were selectively intensified on the modified filter paper, and the sensitivities of determination of them were enhanced.In chapter 3, the determination method of SPE-RTP for fluorene and acenaphthene with beta-cyclodextrin bonded filter paper substrate was developed. The RTP condition of fluorene and acenaphthene on the bonded filter paper was examined in detail. Application of bonded filter paper in SPE and the impact factor of SPE were studied. Under the optimized condition, the enrichment efficiency of fluorene and acenaphthene were more than 100 times. This method was used into determination of fluorene and acenaphthene in environmental water samples, the recovery >90%, the relative standard deviate(RSD) < 7.3%, it indicated that the reliability of the developed method.In chapter 4, the analytical mean of SPE-RTP for carbazole, 7,8-benzoquinoline and phenanthridine with beta-cyclodextrin bonded filter paper was developed. The RTP conditions of carbazole, 7,8-benzoquinoline and phenanthridine and saturated volume and enrichment efficiency on the modified filter paper were examined, and the interferences of PAHs and other NHCs for carbazole, 7,8-benzoquinline and phenanthridind were examined , too. Through a SPE procedure, the limit of detection (LOD) of carbazole, 7,8-be(?)oquinoline and phenanthridine were improved twoorders of magnitude, wh(?) were found to be 9.1×10^-11 , 8.3×10-10and7.8×10-10molL-l respectively. The proposed method was applied for the analysis of water samples. The recoveries of carbazole, 7,8-benzoquinoline and phenanthridine in water samples was from 86.1% to 109.3%.In chapter 5, the application of pattern recognition and principle component analysis in the solid substrate room temperature phosphorescence was studied from both qualitative and quantitative perspective. The performance effective of principle component analysis in the course of dealing with SS-RTP of PAHs and NHCs mixtures was assessed primarily. The results showed that fluorene, acenaphthene, carbazole, 7,8-benzoquinoline and phenanthridine could be identified from arbitrary mixture each other by applying RTF artificial neural net. It indicated that it feasible in the mixtures analysis.