| Camellia oleifera root rot was one kind of root rots which usually jeopardized Camellia oleifera.The root of Camellia oleifera harmed came to rot,which leaded to the death of the plant because of the unusual sorbation of nutiaton and water and brought serious danger to Camellia oleifera production. It has been one of limiting factors to Sustainable Development of Camellia oleifera.In this paper, Camellia oleifera root rot was identified and biological characteristics,nested PCR disease dianosis techolgoy were studied.The pathogens of Camellia oleifera root rot had been separated and identified.Collected 14 species plant culivars infected Camellia oleifera root rot and only one pathogen was isolated.The isolate was inoculated the healthy plant in two means.The results showed that the plant had the same symptom with the speciemen which had gathered from filed.Then isolated from the plant agina,the fungi were were the same pathogen which had isolated from specimen, the pathogenic fungi had strong pathogenicity. Morphological characteristics were expatiated and sequenced the strain of the rDNA internal transcribed spacer (ITS). The homologous strain in the Genbank were download, molecular phylogenetic tree was constructed. Based on morphological characteristics and molecular phylogenetic tree, it can be concluded that the pathogens is Fusarium moniliform.The pathogen's characterization had been studied.It showed that the optimum temperature and pH for mycelial growth of the pathogen were 25℃and pH 5-7.Different lighting time had no significant effect on mycelium growth of Fusarium moniliform.Condia could not germinate if the relative humidity was lower than 90%,the geimation rate was the higest in the water drop.The lethal temperature was 60℃,10minutes, Starch is the best carbon nutrient,NH4Cl is the best nitrogen nutrient.Rapid and accurate detection of the pathogenic of Root Rot of Camellia oleifera diseased plant tissues was developed in the study. Based on differences in internal transcribed spacer(ITS) sequences of Fusarium., a pair of species-specific primers, G1/G2 was synthesized. The others species of Fusarium were used to test the specificity of the primers. G1/G2 amplified only a unique 400 bp band from CSUFT070109 strain. The detection sensitivity with G1/G2 was 1 pg of genomic DNA in 25μL recation solution. A nested PCR procedure using ITS1/ITS4 as the first-round primers and followed with G1/G2 increased detection sensitivity 10000-fold to 100 ag. The results suggested that the assay detected the pathogen more rapidly and accurately than the standard isolation methods.The PCR-based methods developed here could simplify both plant disease diagnosis and pathogen monitoring, as well as guiding plant disease management.In this study,we identified the pathogen causing Camellia oleifera root rot as Fusarium moniliform.providing information to protect and control Camellia oleifera correctly.this pathogen's characterization was studied to make clear its surrounding and make proper programme how to control root rot efficiently. Besides,through the nested PCR dianosis techololigy studied we could also established one efficient methods for early disease dianosis,pest control,monitoring and forecasting of Camellia oleifera root rot... |
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