| Polysaccharide is the sugar chain which unifies through the sugar glucoside key, at least more than 10 monosaccharide composition of the aggregate polymer carbohydrates. Polysaccharide widely was found in higher plants, animals and microorganisms cell wall, is one of four big basic composition materials of life and closely related to the maintenance of life Because the structure of the polysaccharide complex and difficult to control, so the research has lagged behind polysaccharide proteins, nucleic acids and lipids. With the development of molecular biology, people had learned gradually about the activities of polysaccharides involved in the mechanism of cell function, and realized gradually the important biological function to the polysaccharide and the compound. In recent years a large number of pharmacological and clinical study found that plant polysaccharides is an immunomodulatory agent, suppressesed the tumor to grow, and improve immune function and so on, and no toxic side effects on normal cells. Specially the water-soluble polysaccharide which separates from the traditional Chinese medicine has a very important physiology and pharmacological action. Liliaceae Ophiopogon root perennial herbs, the main chemical composition of flavonoids, saponins, polysaccharides, amino acids and trace elements, with myocardial ischemia, anti-arrhythmia, anti-aging and anti-oxidation, anti-tumor effect on the immune The role of the system, lowering blood sugar and so on. Ophiopogon jaonicus is Root tuber of Herb to Liliaceae Perennial. The main chemical composition is flavonoids, saponins, polysaccharides, amino acids and trace elements, has the anti-cardiac muscle ischemia function, the anti-arrythmia, anti-senile and the oxidation resistance function, the anti-tumor function, to immune system's function, falls the blood sugar function and so on.China doctor thought that it has raises promotes saliva or body fluids, Moisture lungs and the efficacy of pure heart, Usually used for Dryness of dry cough, consumptive cough, Tianjin injury thirst, upset, insomnia, constipation, dry constipation and so on sickness.Ophiopogon in China mainly in Sichuan, Hubei, Zhejiang. The more research about Ophiopogon mainly aimed at Zhejiang's.but in recent years, Zhejiang Ophiopogon less in the market, it is essential to the mainstream of the market research necessary Radix, in the hope of guiding the clinical application of a certain significance. In this paper,The Object of study.is the genus of Liliaceae Hubei Liriope Ophiopogon tuber dry.Different components within Ophiopogon had the different biological activities, with the recent in-depth study of the polysaccharides found that polysaccharides have anti-aging, anti-allergy, hypoglycemia, cerebral ischemia protection, enhance immune function and A variety of biological activities This article research System's about Hubei Extraction of polysaccharides and their structure and antioxidant activity.(1) Ophiopogon japonicus polysaccharide extraction of total optimization:in order to Optimization extraction the technique of Ophiopogon japonicus, Uses the response surface analytic method in the single factor's foundation,research the influence for the extraction rate,taken the total polysaccharide extraction rate as the target, the inspection supersonic time, the supersonic power, the material fluid compares as Fundamental factor. The results showed that time, power, solid to liquid ratio all play a role in the experiment, The effect on the extraction rate:Liquid ratio> Ultrasonic time> Ultrasonic power, The best technological conditions are Extraction time18.98min, Supersonic power354W, Liquid ratiol:24.77,Theoretical predictions and experimental results of the extraction rate of deviation is 0.0604%.It provided the Basic experimenta evidence for extraction the active ingredients of the polysaccharides maximum and reasonable development and useful.(2) Hot water formulation and ultrasonic about polysaccharides structure's contrast, Extracted polysaccharides according to the most optimal combination of access, obtaining winter polysaccharide (WBP) and ultra-Ti Mai Dong polysaccharide (SBP). observated surface contours By gas chromatography monosaccharide composition, infrared spectrum analysis functional group, environmental scanning electron microscope and atomic force microscopy The results showed that WBP and SBP has the same monosaccharide composition, are included arabinose, mannose, glucose and galactose, The infrared spectrum analysis result showed that supersonic causes the polysaccharide functional group to change, The environment scanning electronic microscope's result showed that polysaccharide's sheet structure which extracted by ultrasonic smaller in varying degrees, Atomic force microscope analysis shows that ultrasound wave make chains of sugar molecules to smaller, The results show that the ultrasonic treatment does not affect the monosaccharide composition of polysaccharides but will change the functional groups and micro structure(3) Activeness contrast of oxidation resistance about polysaccharide by Hot water formulation and ultrasonic wave formulation obtained Under vitro chemical simulation conditions Measured the total reduction capacity of WBP and SBP, To hydroxyl free radical scavenging action,To O-2 Scavenging action and Inhibition cause by Fe2 Induced inhibition of lipid peroxidation. In vitro experiments showed that the antioxidant activity of polysaccharide obtained by ultrasonic wave treatment were improved, in addition to superoxide anion (O-2) scavenging Loss. |
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