The Study Of Ustilago Maydis And Protein Molecules With Atomic Force Microscopy

Comprehension of the cell surface topography structure is important foundation to further understand the cell function and biological phenomena. Different tools and technology play an important role in researches. Atomic force microscope (AFM) is suitable for high-resolution imaging. Simple preparation for samples makes fewer changes on biological samples and the morphological structure of biological cells could be observed in physiological conditions.The surface topography structure of Ustilago maydis haploid cells were characterized by AFM and optical microscopy. The effects of antibacterial agent hygromycin and Carboxin on topography structure of Ustilago maydis cells were observed. The interactions among proteins were also investigated by single molecule force spectroscopy. The content of article mainly includes the following three aspects.1. Morpholoical changes of Ustilago maydis haploid and Ustilago maydis in long-term air exposure be observed via AFM. Morphological changes of combination tube in haploid and its dozen-nanometer-level morphology could be observed in AFM.2. The effects of antibacterial agent hygromycin and Carboxin on morphology of Ustilago maydis were investigated by AFM. The results showed that Carboxin could not only inhibit the growth of Ustilago maydis, but also induced pits in cell surface. It was found that cell surface of Ustilago maydis become smoother, less rough, and no pits appeared after adding hygromycin.3. The interactions between spore coat proteins were studied by single molecule force spectroscopy. The interaction force between CotE is 49.4 pN at loading rate of 3160 pNs-1. The dissociation rate constant is about 0.146s-1. The dynamic information was obtained.