Atomic Force Microscopy Study On Fungus And Cell Morphology And Dynamics

In this dissertation, the property of surface morphology, Young's modulus and adhesion force were investigated by atomic force microscopy (AFM). The surface morphology of Ustilago maydis and HCT116cell were studied, the surface morphological changes of the cell after drug treatment were observed. And the effects of TNFa or Gefitnib on the surface properties of cell were also observed via single molecule force spectroscopy. The main contents are as follows:1. AFM study of the effect of triadimefon on the surface morphology of Ustilago maydis. First, we investigated the surface morphology of Ustilago maydis in the air, and the effect of triadimefon on Ustilago maydis. Our results demonstrate that triadimefon damaged the structure of Ustilago maydis surface. With the increase of triadimefon concentration, the extent of damage was increased.2. AFM study of the effect of TNFa on the surface morphology and dynamic properties of HCT116cell. After being incubated with20ng/mL TNFa, the shape of the cell was changed and EMT occurred.The Young's modulus of HCT116cell surface was decreased then increased, and adhesion force of tip and cell was increased then decreased.3. AFM study of the effect of gefitnib on the surface morphology and dynamic properties of HCT116cell. Being incubated wth0.5μmol/L gefitnib lead that the growth of cell was inhibited and the adhesion force between the tip and the cell was increased..