The Investigation Of Chemical Composition Extraction And Activity Of Fertility-promoting Intrauterine Infusion Powder (fpp)

Major chemical composition of fertility-promoting intrauterine infusion powder(FPP) was extracted by percolation method and nonpolar solvent, polar solvent, powerful polar solvent was used in turns. The utility active solvent section was got by testing the increasing weight of the hystera seprately, the follicular diameter increasing and the content of the aquadiol in blood serum.Then the extraction technology was optimized and the best extraction condition was definited.All that had been done was to provid theory basing for the investigation of traditional Chinese drug extractive technique, reduce clinical drug consumption and develope new dosage. The article contents as follow:Major active component of chinese tradational medicine compound recipe FPP was screened by the effects of increasing the weight of the hystera and follicular development of the twenty-one days old feminity Mus musculus albus.Of course, the mouse was chosen as the first animal subject and compared the effects of three different solvent extract which contain ligroin extract, alcoho extract and water extract.Among three different solvent extractions, alcoho extract had exceeding significantly effects than the normal group on increasing the weight of the hystera and follicular development(P<0.01).There is no clear differenc between the ligroin extract group, water extract group and normal group.The effects of the alcoho extract showed that lyso-alcohol composition is the arch-active component.The data showed that the flavone and alkaloid were major in alcohol. Icariin was taken as the index, ultrasound-assisted extraction was used on the base of single factor experiment and the best extraction techniques of Flavanone component was selected by orthogonal experiment. The best extraction tec-niques:solvent:60% ethanol, extraction time:25 minitues, extraction times:2 times, and the solid-liquid ratio:1:12;the content of total flavonoids in FPP is 1.236%.Acid-stachydrine was taken as the index, ultrasound-assisted extraction was used on the base of single factor experiment and the best extraction techniques of alkaloidal component was selected by orthogonal experiment.The best extraction techniques:60% ethanol as solvent, solid-liquid ratio:1:10, extraction time:20 minitues, extraction times:3 times; the content of total alkaloids in FPP is 1.148%.The major activity component of FPP was chosen by the animal experiment which was fed the flavone extract and alkaloid extract.The results showed that the flavone extract had much better effects than the alkaloid extract on increasing the weight of the hystera and follicular development of the twenty-one days old feminity Mus musculus albus.All these showed the flavone are the major active component of the traditional Chinese medicine prescription.