Study On Some Biological Characteristics Of Staphylococcus Xylose From Bovine Mastitis And Detection Of Class Ⅰ Integron

This study take part dairy farm clinical mastitis isolated Staphylococcus xylose 65 strain from north of xinjiang, taking convention Physiological and biochemical identification method, API identification, and molecular biology identification sieve 60 strain Staphylococcus xylose.This experiment take pathogenicity experiment, resistant experiment and carry the PCR detection of class I integron, analysis dependability of integron to resistant phenotypic. Consequence:1.The tested strains are staphylocoagulase negative, Heat-resistant nuclease-negative, Catalase and mannitol fermentation positive.8 bacteral strain haveβhemolysis,5 bacteral strain have pathogenicity.2.16SrRNA gene PCR for detection of Staphylococcus xylose,60 strains had been amplificated the same fragment. I had selected typical fragment reclaim, sequence, comparison by ncbi,homology 98.97%.3. The drug sensitivity tests showed that resistant to Sulfadiazine Sodium (drag fast rate 100%),benzylpenicillin potassium (drag fast rate 93.3%), Tetracycline (drag fast rate 90.0%), Gentamicin (drag fast rate 90.0%), streptomycin sulfate (drag fast rate 86.7%), sensitive to Amikacin (drag fast rate 6.7%), Enrofloxac (drag fast rate 10.0%). Experiment strains main drug fast to aminoglycosides, penicillins, sulfamido appearance hadro-resistant.4. This research has detected Staphylococcus xylose class I integrase gene and resistant cassettes from north-territory strain, Analysis of strains carrying integrons and antimicrobial resistance phenotype correlation. Consequence, class I integron-positive strains of resistant strains was significantly higher than negative, susceptibility test discovery, class I integron have correlation to Staphylococcus xylose resistance. Class I integron positive strain facility form resistance to negative strain. Through integron system research, it can comprehend bacterium resistant mechanism.