The Comparison Of Therapeutic Efficacy Between Liposomal Albendazole And Albendazole On Echinococcosis Sheep

To authenticate and compare the therapeutic effect of liposomal Albendazole and Albendazole on Echinococcosis sheep The paper was designed to compare the therapeutic efficacy of liposomal Albendazole and Albendazole on Echinococcosis sheep. After artificial challenge ,the tentative sheep were divided into two groups by different drug, and differential count on sheep peripheral blood leucocyte(acidophilia,neutrophilia,basophilia,granular cell,lymph cell,histoleucocyte)of each group at different time points; to detect CD4+T,CD8+T,B lymph ce ll,glutamate-pyruvate transaminase and total serum protein level by Flow Cytometry(FCM), and analysis the change rule about the index of humoral immunity,mediated immunity of sheep when to cure the echinococcosis by different drug at different time , compare the therapeutic efficacy of liposomal Albendazole and Albendazole on Echinococcosis sheep, at the same time , make manufacture and observe histopathologic slide, contrast the therapeutic efficacy of two drug and provide base for clinical medication.In the first experiment, we infected 10 sheep artificially with the eggs of Echinococcus granulosuse ,which were obtained from the experinment dogs. The day of inoculation is 0d, then abruptio blood serum from sheep whole blood on 45d and 60d,we examined the infection of E. granulosus sheep quickly and exactly by the ELISA diagnose Kit . we infected 6 sheep artificially. They were divided into 2 groups with 3sheep of each one: one group was orally given Albendazole and the other was given liposomal Albendazole for 15d and 30d,then collect blood sample. This study evaluates the therapeutic effect through the blood Immunization indicators such as:CD4+T lymphocytes, CD8+T lymphocytes, B lymphocytes, total leukocytes, lymphocytes, neutrophils, as well as alanine aminotransferase and serum total protein level by Flow cytometry. Results showed that the administration of 15 days later,CD8+T lymphocyte level of ABZ group was significantly higher than that of L-ABZ group , (P<0.05); the neutrophils of L-ABZ Group was significantly higher than that of ABZ group (P<0.05); 30 days later, the serum total protein level of ABZ were significantly higher than L-ABZ group (P<0.05). Before and after administration, there was no significant difference in CD4+T lymphocytes, B lymphocytes, total leukocytes, lymphocytes and alanine aminotransferase between ABZ and L-ABZ (P>0.05),which indicated that the effect of L-ABZ was superior to that of ABZ ,playing an energetic role in curing Ovis aries echinococcosis and calcifying Ves.The second experiment: the homeochronous artificial infection sheep were divided into 2 groups with 3 sheep for each one:Po-ABZ and Po-L-ABZ group by mouth for 30d . After the administration of 30 days Continuously,we observed the the change of the hydatid's shape and pathologic histology and evaluated the therapeutic effect. The result indicated that therapeutic effect in liposomal Albendazole group is better than Albendazole group.