Barley Btr Gene Transfer Research

Study on Transferring Btr Gene into Immature Embryos of Barley ABSTRACT Barley quality breeding through biotechnology is one of the popular methods in barley breeding research field. By selecting the optimum condition of tissue culture, the results showed that ABA O.5mg/L, CH300rng/L and 2,4-D 2mg/L added in MS, a basic medium, can control the pre-germination of barley immature embryos and promote calli's formation. ZTO. 1 mg/L and IAA 1 .Omgl in differentiation medium, can improve calli's differentiation. Btr gene, improving the beer productivity of barley and Bar gene, resisting PPT, were co-transferred into the barley immature embryos by gene-gun. 17 regeneration plantlets were gotten. By PPT-test, Bar gene were transferred into 7 plantlets and Btr interested gene into 2 plantlets by special PCR-test.