Research On The Tissue Culture Technology Of Chinese Cabbage Immature Embryos

Chinese Cabbage (Brassica campestris ssp.pekinensis) is a cruciferous Brassica(Cruciferae)(Brassica), one or two annual herb, also known as Chinese cabbage,Chinese cabbage, cabbage, Chinese cabbage types and varieties in China is very rich,according to the head can be divided into four types，heading, half-heading，flowerand loose leaf，according to the ecological types can be divided into three types，straight, oval and flat.In this paper, according to the6varieties of Chinese cabbage were embryonicage selection, selection of basic medium, plant growth regulators added test,following the screening test screening culture medium and rooting medium, thefollowing results are obtained:(1) Under aseptic conditions stripping embryo indoor culture in vitro, to test the4d-24d to determine the optimal embryo, embryo age time cabbage young forembryo culture is18d-24d。(2) Using MS,1/2MS, B5three kinds of medium to culture the Chinese cabbageyoung embryo, the best medium is: MS+30g/L sucrose+0.7%agar medium.(3) By adding different concentration of plant growth regulators (6-BA, NAA), fourkinds of different types, cabbage leaf ball obvolvent types, leaf ball shape ofChinese cabbage seedling of immature embryo culture medium induced byhormone levels were selected: medium for Chinese cabbage cabbage folded shelltype for the MS+NAA0.4mg/L+6-BA (0.5-1.0) mg/L+30g/L sucrose+0.7%agar; medium for cabbage folded inverted cone type in Chinese cabbage isMS+NAA (0.4-0.6) mg/L+6-BA (0.5-1.5) mg/L+30g/L sucrose+0.7%agar;medium for heading just straight type cabbage MS+NAA (0.4-0.6) mg/L+6-BA(1.0-1.5) mg/L+30g/L; suitable for cabbage encircle the oval, half a cabbageturn heart short cylinder and half heading over the heart tube type cabbagemedium was MS+NAA0.6mg/L+6-BA1.5mg/L+30g/L sucrose+0.7%agar.(4) Through the subculture, and gives6different genotypes of Chinese cabbageshoots subculture medium. For cabbage he shells, cabbage folded inverted conetype in folded, oval, straight in just four types of Chinese cabbage in the subculture medium is MS+NAA0.6mg/L+6-BA1.5mg/L+30g/L sucrose+0.7%agar. Medium for over half of the heart short tube and half heading overthe heart tube type in Chinese cabbage is MS+NAA0.6mg/L+6-BA0.5mg/L+30g/L sucrose+0.7%agar.(5) Through the rooting medium screening test, medium the cabbage+encircle+shells type:1/2MS+NAA0.3mg/L+30g/L sucrose+0.7%agar; cabbage+folded+inverted cone, cabbage+encircle+oval, cabbage+encircle+straight,half cabbage+turn heart+short tube, half cabbage+turn heart+longcylindrical rooting culture medium formula is:1/2MS+NAA0.4mg/L+30g/Lsucrose+0.7%agar.