| Boer goat, being from South Africa, is considered to be the premier goat meat producing breed. It owns so many good traits as large form size, high adaptability to environmental conditions and high fertility, and it play the important role in goat breeding process. But the number of Boer goat holds back the development of the meat goat industry. Now superovulation and embryo transferring techniques are used to solve this problem. But the results of it are various and no succeeding progress is formed. In our research, we focused our study on such aspects as superovulation, laparoscopic inspecting, embryo recovering and embryo transferring , and tried to establish a good procedure of embryo transfer. These will make the base to the next research in nuclear transferring, embryo splitting, in vitro fertilization and transgenic animal production.In this paper, we studied the techniques of embryo transferring at first. In the embryo transfer experiments on Boer goat, three methods were used. In method one, the donor goats were plugged with progestagen sponge in DO, and at the same time, estradiol was injected. Then a uniform cohort of follicles is recruited by the administration of FSH eight times beginning at D12 (total does of 280IU), the progestagen sponge were removed in D13. In the method two, the donor goats were administrated with PG (does, 0.6ml per goat), then FSH was used for superovulation beginning at D15(total does of 280IU), PG was admistrated at D17(does,0.6ml per goat), FSH being used of method one and two was from native .In the method three, CIDR was plugged to improve superovulation at DO, then at D12 fresh CIDR was used to change the old one. In the afternoon of D12, The donor was administrated with FSH for superovulation. In this method , two kinds of FSH were used ,one is from Australia (total dose 25NIH per goat),the other from Canada (total dose 300mg per goat). Laparoscopic was used to check the status of donor's ovary after superovulation. We found that the result of method one and two is so poor, and FSH we used may be with this result. In the method three, 5 donors were superovulated with FSH from Canada. Fertilization failure was found in three goats. We recovered-6-20 embryos from the other two, and then we transfer these embryos into native goat. 5 months later, 2 baby goats were born. We can conclude that the fertilization rate maybe with the dose of FSH used for superovulation.7 donors were superovulated with FSH from Australia, and 91 embryos were recovered, then we transferred these embryos into 54 recipients. 40 Boer goats were born, and the birth rate was 43.9%.In the second experiment, we tried to use the gamete intrafallopian transfer technique to solve the infertile problem in the goats. 9 oocytes were recovered from 2 donor Boer goats and they were transferred into 4 recipients, the result shows no recipient of it was pregnant. The other 30 oocytes were recovered from native goats and hybrid goats. They were transferred into 8 recipients, 3 of them were pregnant, and 5 goats were born, the development rate of oocytes was 13%. From this experiment, the possibility of GIFT in the goat is proved.No gamete developed into baby Boer goat in the last GIFT procedure. This may has the relationship with the status of oocytes maturation. So we cultured the oocytes before transfer, and the culture system was TCM199 (1 Dug/ml FSH +20ug/ml LH +lug/m 1 1?P-E2+10% EGS). 9 oocytes were recovered from two donors .26 hours later, the cumulus cells of six oocytes spended well and We defined they were matured. Sperm were capacitated in the capacitation medium with heparin and coffein. Then the matured oocytes and capacitation sperm were transferred into 4 recipients fallopians . Now, two of them were pregnant. So we can conclude that the oocytes maturation can improve the success rate of GIFT procedure.In our research, we established a series techniques about embryo transfer and gamete intrafallopian transfer. These can serve for embryo transfer in large scale and will provide... |
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