Guizhou Quality And High Yield Poria Strains Isolated Purification Filter And Applied Research

The drying sclerotium of fungus Wolfiporia cocos (Wolfiporia cocos(Schwein.)Ryvarden et Gilbn.) is a traditional costful medicine in China. Wolfiporia cocos is promissing because of its high medical value, excellent nutritional value, unique healthy Function and considerable economic value.Organisation separation of sclerotium of wild Wolfiporia cocos from guizhou and Wolfiporia cocos of artificial cultivation were done, micro-morphological observation and observation by fluorescent HOECHST 33258 Stain wrere done in this paper. The optimum medium of female parent and mother seed were screened out in this experiment. We screened out the 4 trains with excellent quality and high yield by primary screeningtest of hyphae growth and secondary screening of protospecies cultivation from 13 trains of wild Wolfiporia cocos from guizhou and Wolfiporia cocos of Artificial cultivation, 6 trains collected by CCTCC and 4 main cultivated trains in our country. In outdoo rexamination cultivation, mycelial germination and growth of the 4 trains is good, the percentage of sclerotium forming from hyphae of wolfiporia cocos is 100%, all target aims were superior to the control strain, No.5. The results of identification for emphytic character showed that emphytic characters were stabile. We propose the screening model of the high yielding and good quality of Wolfiporia cocos on the base of above-mentioned test to offer the realistic basis for the selectiond and production study of Wolfiporia cocos.We determined the growth rate of test organisms on boxwood dust culture medium and the activity of CMCase secreted to boxwood dust culture medium and analyze the correlation between growth rate and activity of CMCase. The experimental results indicated there was positive correlation between growth rate and activity of CMCase (r =0.951977)and the activity of FPA is negative.17 primers with clear bands ,good polymorphism and repeating were screened from 41 primers through analysis of 23 strains by PCR. Furthermore genetic analysis and identification about 10 exotic tuckahoe strains and separation strains of tuckahoe in guizhou were done in this study according to RAPD method, building unrooted tree and analysising of data to analyse genetic relationship primarily. The result show that there are extremely close genetic relationship between wolfiporia cocos from different area. The cluster results were basically in consistence with the identification results of morphological character of 23 trains. This part show the clustering analysisis of wild Wolfiporia cocos is conducive to the research of genetic relationship and nucleotide homology and offer molecular biology evidence for the classification and identification of Wolfiporia cocos.Results of inoculum preservation show that the culture preservation with glycerol and culture preservation with bevel have same perfect effect through comparison of colonial morphology,mycelium biomass and growth rhythm on fluid nutrient medium and solid culture medium. This conclusion accords with results of morphological character identification of 23 strains.