Turn With Ipt-bar-transgenic Rice Genetics And Cold Resistance

Under the conditions of isolation in the field,through a number of research groups from generation to generation,analysis of the ipt,bar gene in the inheritance of all groups,ipt plants positive and negative plants' cold resistance;studied on the transfer ipt-bar dual gene R527 agronomic traits,analysis the difference of cold resistance between transgenic hybrid rice with the CK,the results showed that:1.The separation ratio of the Ipt gene in B2F2 and B2F3 groups of(ipt-bar dual transgenic Ey 105×Shuhui 527)×Shuhui 527],T2 and T3 groups of ipt-bar dual transgenic Shuhui 527 was3:1,met the law of Mendel,the performance was single-gene,while in B2F2 and B2F3 groups of(ipt-bar dual transgenic of Ey 105×Mianhui 725)×Mianhui725,the separation ratio did not met the laws of Mendel.2.The separation of the Bar gene did not showed the same rule,and most do not meet the ratio of 3:1,just met the ratio of 3:1 in the generation groups T2 and T3 groups of ipt-bar dual transgenic Shuhui 527,showed as heredity of a pair of dominant genes.3.In all the generation groups which were studied,under the cold treatment of low-temperature at booting-flowering stage,compared the positive plant of ipt transgenic with CK,the effect of low-temperature on conductivity and chloroplast a/ b of positive was less than CK,and the content of proline and sugar was more than CK,the effect on the seed-setting rate was less than CK.The principal component analysis of conductivity,Chlorophyll a/b and the content of praline and sugar showed that the first principal component was seed-setting rate,the second was the Chlorophyll a/b and the third was the sugar content.4.Under the condition of cold treatment,the plants of ipt-bar transgenic showed a greater coefficient of variation and irregular on the agronomic traits such as the amount of effective ears and seeds.5.Under the cold treatment of 17℃-low-temperature at booting-flowering stage,the seed-setting rate of the plants of ipt-bar transgenic was 80.08%,was Significantly higher than that of CK,which was just 28.74%,showed stronger cold resistance;by analysising the agronomic traits,the results showed that the main factors which affected the yield of the single plant of transgenic Jinyou527 were grain weight and effective ear.