Peanuts And Soil - Determination Of Daminozide, Bhc And Acetochlor And Its Metabolites With The Study

The presence of pesticide residues in food is crucial to human's life. It is one of the most serious problems concerning food safety that has attracted great attention of related governments and organizations. Pesticide residues in peanut have higher toxicity than those in other food. However, there are many lipids and other substrates in peanut, which disturb the detection of pesticides sensitively by traditional method. Thus, it is of significance to develop new analytical methods for the detection of pesticide residues in peanut and relevant food. The work focused on the solving of this problem and a facile analytical method was developed and studied to detect multiple pesticide residues in peanut samples. The new procedure involves pretreatment, such as extraction with acetonitrile and purification with solid-phase extraction (SPE), and chromatograph analyses, which is rapid, convenient and economical compared with the traditional ones. The content and results of the work are as follows:1,According the detection of daminozide , the analytical method for the detection of pesticide residues in peanut was established. The content of pesticide residues in soil was determined using this method. The metabolizable process and products of daminozide were investigated and the corresponding analytical procedure was developed accordingly. The gas chromatography analyses (GC) and pretreatment conditions including extraction, hydrolyzation, and purification were optimized for the detection of total residue content of daminozide and its metabolizable products. The optimal condition was utilized and the recoveries of daminozide from peanut and soil were evaluated to be ranging from 84.7 to 98.6%, and the limits of quantification below 0.0045 mg/kg were achieved.2,The gas chromatography analyses (GC) and pretreatment conditions were optimized for the detection of total residue content of hexachlorocyolohexane (BHC) , acetochlor and its metabolizable products. Using the optimal condition, the recoveries from peanut and soil were evaluated to be ranging from 85.3 to 96.8%, and the limits of quantification below 0.0038 mg/kg were achieved, which fulfilled the practical application well.3,The liquid chromatography analyses (LC) and pretreatment conditions were optimized for the detection of daminozide and its final metabolizable products. The optimal condition involves pretreatment, such as extraction with acetonitrile, washed with saturated NaCl aqueous solution and purification with PSA and SPE, and LC analyses, namely Symetryshelid C18 column (3.9*150mm 5μm), acetonitrile/25mM H3PO4 as mobile phase, detected with diode array detector. The recoveries from peanut were evaluated to be ranging from 78.2 to 98.2% (variation coefficient: 2.42-11.34%), and the limits of quantification below 0.02 mg/kg were achieved. The recoveries from soil were evaluated to be ranging from 77.2 to 93.5% (variation coefficient: 2.97-7.56%), and the limits of quantification below 0.004 mg/kg were achieved.