Study Of Genetic Diversity. Tibet Chicken Of Mhc B-g

Chicken major histocompatibility complex (Major histocompatibility complex, MHC) BG poultry specific gene, encoding it with the type of MHCâ… andâ…¡antigen closely linked genes, located in the middle on the 16th minute chromosome; from their encoding highly polymorphic BG antigens of the MHC antigen is an integral part of one of the chicken MHCâ…£as antigen, also known as BG molecules are immunoglobulin superfamily members of a special.GenBank under the red jungle fowl MHC BG gene sequences (landing No.: NC₀06103.2) designed nine pairs of primers for three species of chicken for a total of 72 chicken sequencing analysis, to find SNPs. Resulted in the discovery of 17 single nucleotide polymorphisms, or SNPs.That is, primers P1 gene sequence of the 177 C→T, 345 bit insertion, 511 C→T, 547 bit T→C; primer P2 gene sequence of the 2053 C→T, 2035-bit and 2032 T→C and T→G; primer P3 sequence the entire genome of the 3108 T→C, 2889 bit T→A, 2866-bit and 2864 A→G and C→G, 2822-bit and 2816 C→T and G→C; P8 primer the entire gene sequence of the 6406 G→C, 6329 bit G→A, 6289 and 6285 A→G and G→T.Respectively before and after 17 mutation points of transcriptional regulation factor for the prediction, primer P1 was found that the entire gene sequence occurred in the first 547 base pairs of the T→C conversion can be predicted a reduction of two regulatory elements of the binding sites are transcription factor HSF1 (forecast higher score 87.0) and the transcription factor HSF2 (a higher score of 85 forecasts.9); P2 primer whole genome nucleotide sequence of the first places respectively 2053,2035,2032 the T→C, A→G, A→C conversion can predict an increase of two regulatory elements of the binding sites, which are transcription factor MZF1 (the highest prediction score 94.8) and the transcription factor E47 (forecast higher score 88.5); primer P3 sequence the entire gene, respectively, the first 3108 base pairs of the A→T occurred after the conversion can be predicted to reduce to a regulatory element binding point and a predicted increase in the regulatory element binding sites, which are transcription factor GATA-1 (high prediction score 85.3) and the transcription factor GATA-1 (a higher score of 91 forecasts.8), 2889,2866,2864, respectively, occurred in the base spaces A→T, T→A, G→C conversion can be predicted a reduction of two regulatory elements of the binding sites, which are transcription factor AML-1a ( forecast a higher score 87.4) and the transcription factor cdxA (forecast higher score 85.0), the first base position, respectively, 2822,2816 occurred G→A, C→G conversion after a predictable increase in the binding of regulatory elements point of transcription factor SRY (higher prediction score of 90.0); primer P8 gene sequence 6406 base pairs, respectively, the first occurrence of the C→G added a conversion and a reduction of one could forecast that the regulatory element binding sites, which are transcription factor OCT-1 (the highest prediction scores 91.8) and the transcription factor OCT-1 (high prediction score 85.7). Other point mutations did not lead to any potential regulatory elements and protein binding site changes.