Hmw-gs Analysis Of Wheat Xiao Yan81 Implanted By Low Energy N~+ Ion-beam

XiaoYan81 dry seeds were implanted by low energy N⁺ ion-beam(with 30 KeV energy),and then the main agronomic properties of M₂ generation plants in the field were observed.The results showed that many properties were mutanted such as plant height,ear type,wheat awn,protein content and yield traits etc after implantation.We observed there were 9 mutant types of ear,and others such as purple stem,thick stem,phimosis, early-maturing,infertility,disease susceptibility and easy-lodging.The correlation of Agronomic to Quality Characters in M₂ generation of wheat XiaoYan81 implanted by low energy N⁺ ion-beam was also analized.The results indicated that protein content of wheat grain were significantly negative correlation with the 1000-grain weight,harvest index and had not correlattion with tiller number.The 1000-grain weight was negative correlated with tiller number.Harvest index was significantly positive correlation with the 1000-grain weight,and had not correlation with tiller number.The variation and composition of high-molecula-weight glutenin subunits (HMW-GS) of 3081 M₂ seeds of wheat xiaoyan81 implanted by two radiation doses were analyzed by using SDS-PAGE.Six missing mutant types of HMW-GS were obtained. They are 1Ax1 missing mutant,1Bx14+1By15 missing mutant,1Dx2+1Dy12 missing mutant,1Dy12 missing mutant,1By15 missing mutant and 1By15+1Dy12 missing mutant. The results also indicated that HMW-GS mutants induced by N⁺ implantation had dose effect and subunits sites in different chromosomes had different sensitive degree to N⁺.The mutant frequency of subunit sites was Glu-1A＞Glu-1B＞Glu-1D,and it's same to both doses.The same analysis was done to M₃ generations,and the results showed that 4 kinds of HMW-GS missing mutant were found.They were 1Ax1 missing mutant,1Bx14+1By15 missing mutant,1Dx2+1Dy12 missing mutant and 1Dy12 missing mutant.So these four kinds of mutant could be inherited,its genetic variation rate was 23.2%.The swelling index of glutenin(SIG) in flour from CK,Chinese Spring,1Ax1 missing mutant and 1Bx14+1By15 missing mutant were detected.The results show that the quality of mutants were generally lower than wild-type.SIG also had relevance to the number and types of subunits,the less subunits had smaller SIG..subunit 7+8 was more important to the quality of wheat than subunit 14+15.