The Study Of Neospora Caninum Vaccine On Immuno-effection Of Goat

Neosporosis is a kind of protozoal disease caused by neospora canium parasitizes in various kinds of livestock such as dog, cattle, horse, and sheep. A main reason of cow abortion is neosporosis, which spread widely all over the world, the abortion of cow caused huge economic loses for cattle breeding. Along with the deeply studies and elevated study method, the immunology, molecular biology and biotechnology and other advanced technologies were taken used to study the immunoreaction for host anti-neospora caninum and the vaccines for preventing neospora caninum, many advancements received.In order to comprehend the immuno-effective of neospora caninum vaccine preferably, a large number of neospora caninum was cultured in this test,then the antigen of neospora caninum was extracted by Freeze+thaw, the neospora caninum vaccine was prepared by mixing the antigen and adjuvant. We took use of sheeps to have the immuno-effective test.IFAT was taken to monitor the antibody level of cow serum after immunization, the different antibody titers were determined, including immunization before gestation and after gestation, evaluating the immuno-effective of vaccine immunization for neospora caninum, which established the foundation for evaluating immuno-effective of vaccine for neospora caninum.The results showed that the first group, two weeks after the first immunization, the majority of goats to produce antibodies titer 1:400. After second immunization, antibody titer was significantly rised, in the sixth week, the highest antibody titer was 1:6 400, after the artificial infection the goat antibody titer increased suddenly, the highest antibody titer reached 1:12800.The second group after two weeks of the first immunization, most of the goats would produce antibodies, antibody titer of about 1:400. After second immunization, antibody titer was significantly rised, in the sixth week, the highest antibody titer was 1:6400, after the artificial infection the goat antibody titer increased significant, the highest antibody titer reached 1:12800, and the antibody titer maintain higher between the sixth and eighth weeks after the artifical infection. With the artificial infection in control group, goat antibody titer increased quickly, after a week the all of the goats in control group had produced antibodies, antibody titer of 1:200, and after the third week, the high titer was 1:1600.Artificial infection test results show that the first group during pregnancy before the immunization, the protection rate of 71.4%(5/7); the second group after the immunization during pregnancy, the second group of vaccine protection rate of 57.1% (4/7). The third group was not immunized control group, the protection rate was 0, and experimental results show that the neospora caninum vaccine has reliable protection against goat abortions.