| The research of fumaric acid, malic acid and other organic acids on the ruminant animal become more and more. In this paper, the test was used the Yanbian Semi-fuzz sheep installed rumen fistula as the test animals to study the effect of organic acids on rumen fermentation function and feed conversion ratio, through, the rumen disappearance rate, rumen fermentation characteristics, microbial ecology and nutrient digestion rate study, the best organic acid and its appropriate content for Yanbian Semi-fuzz sheep was selected through the determination of rumen disappearance rate, rumen fermentation characteristics, microbial ecology and nutrient digestibility, these provide scientific basis for the application of organic acids on the Yanbian Semi-fuzz sheep.This design was used 3×3 Latin square method and three Yanbian Semi-fuzz sheep installed rumen fistula were as the experimental animals. The whole experiment was divided into two parts:1, The effect of fumaric acid, malic acid on rumen fermentation and disappearance rate.The test was divided into the fumaric acid group added 13g/d, the malic acid group added 10g/d and the control group without any treatment. The rumen fermentation characteristics and feed degradation rates were determined mainly.The results showed as following:After the fumaric acid and malic acid were fed respectively, the brewer's grain dry matter (DM) degradation rates in rumen of the fumaric acid group and the malic acid group after 12h were significantly higher than that of the control group (P<0.05); while after 24h the rates of three groups were significant difference (P<0.05), the rate of the fumaric acid group was significantly higher than that of the malic acid group (P<0.05).The organic matter (OM) of two treatment groups after 8h,12h,48h were significantly higher than that of the control group (P<0.01). After fed in the rumen, the trend of pH, NH3-N levels within 10h of two treatment groups were raised compared with the control group, but the differences were not significant. The number of rumen protozoa after 4h and 6h in fumaric acid group was significantly higher than the other two groups (P <0.05). The blood serum urea nitrogen (BUN), aspartate transaminase (GOT), glutamyl-GGT (GGT) and other biochemical indicators in the various time points after fed were not significantly different, the average differences were not significant.2, The effect of different content of fumaric acid on the Yanbian Semi-fuzz sheep rumen fermentation and digestibilityAccording to the different levels added of the fumaric acid, the test was divided into three experimental groups, which the first group was added 6g/d, while the second group 12g/d, the third group 18g/d. The rumen fermentation and wholly-intestinal digestibility were carried out. The results were indicated that:The rumen pH values in the 4h of the 3rd group was significantly higher than the 1st group (P<0.05). After 2h,4h, the fumaric acid was fed, the rumen NH3-N concentration of the 3rd and 2nd group were significantly higher than that of the 1st group (P<0.05). The feed digestibility of DM, OM, CP, EE, NDF, ADF in the 3rd group was significantly higher than that in the 1st group (P<0.05), meantime, the CP digestibility in the 2nd group was significantly higher than that in the 1st group (P<0.05). For the CMCase activity within 10h after the fumaric acid was fed, three groups were no significant difference. After 2,4 h the number of rumen protozoa of the 3rd group was significantly higher than the 1st group (P<0.05), while other time points no significant difference in three groups, the average term of the 3rd group was significantly higher than t the 1st group(P<0.05). The alkaline phosphatase, total cholesterol, triglycerides, creatinine, glucose, blood urea nitrogen and other indicators of three test groups were not significantly different (P> 0.05). |
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