Effect Of Tfl On Trail In Rat Model Of Hepatic Fibrosis

Objective To study the effect of total flavone of Litchi Chinensis Sonn on anti-hepatic fibrosis in bile duct occlusion rat model and its mechanism.Methods 100 male SD rats were randomly divided into 5 groups, each 20,including sham-operated control group,fibrosis model group,silymarin treated group (50mg/kg·d),TFL high-dose treated group(200mg/kg·d) and TFL low-dose treated group(100mg/kg·d). Preparation of liver fibrosis was induced by bile duct ligation in rats. Four weeks later the rats were sacrificed, serum and liver tissue were taken for testing;The levels of TRAIL in serum were inspected by enzyme-linked immunosorbent assay (ELISA); The levels of Hyaluronic acid(HA) and Laminin(LN) and procollagen typeⅢ(PCⅢ) in serum were detected through radioimmunoassay (RIA);Liver tissues were stained by routine HE stain to assess the degree of liver fibrosis in rats;The expression of TRAIL in liver tissue was assessed by means of immunohistochemistry called Envision.Results Four weeks later,The liver tissue of TFL high-dose treated group and the silymarin group have shown significant improvement on the degree of liver fibrosis.For the levels of TRAIL in serum, TFL high-dose treated group and sham -operated control group and the silymarin-treated group were significantly lower than that in the model group(p<0.05),however there was no significant difference among three groups(p>0.05).Meanwhile,there was no significant difference between TFL low-dose treated group and the model group(p>0.05).The levels of TRAIL in serum are correlated with the expression of TRAIL in liver tissue.The levels of TRAIL in serum are positively related to the levels of HA and LN and PCⅢin serum.Conclusion TRAIL promotes the development of liver fibrosis. TFL may improve the degree of liver fibrosis.Its mechanism may be related to inhibiting the overexpression of TRAIL in liver tissue.