Experiment Of Transplanting Human Umb-ilical Cord Mesenehymal Stem Cells To Regenerate And Repair Acute-tubular Necrosis

Objective:To observe the distribution, chemo taxis and repairation role of human umbilical cord mesenchymal stem cells in the animals of acute tubular necrosis.Methods:1.Cultured and expanded the human umbilical cord mesenchymal stem cells in vitro,labeled the cells with DAPI before transplantation.2.Made ATN models:given 0.2% HgCl2,7mg/kg by intramuscular injection to establish model of Acute tubular necrosis; injected the total dose of 900mg/kg gentamicin, for the first time injected 300mg/kg,200mg/kg was injected after 12h, and injected 200mg/kg subcutaneous twice at intervals of 24h, within 3 days.3.Transplanted human umbilical cord mesenchymal stem cells:By peripheral veins injection and by femoral artery intervention.4.Detected following parameters:①Traced the cells homing:observed the DAPI positive cells under fluorescence microscope.②Detectde the function of renal tubule:BUN,SCr,urine protein etc.③observed the renal pathologic Change by HE and PAS staining.④Detected some relative cytokines:iNOS by immunohistochemistry.Results:1.Gentamicin and HgCl2 could make ATN model successfully.2.The control group did not have any umbilical cord mesenchymal stem cells in the kidneys.3.For animals with acute tubular necrosis,DAPI positive cells could be detected in the tubule at each time of 5 points after transplantaion, liver and pancreas had no human umbilical cord mesenchymal stem cells existed;The mortality rate lowered obviously;The renal function ameliorated visiblely;The renal pathologic change relieved significantly;proinflammatory cytokine iNOS was down-regulated.Conclusion:1.The transplanted human umbilical cord mesenchymal stem cells can survive in the kidneys which experienced the development of acute renal tubular necrosis, the mortality rate lowered obviously,the renal function ameliorated visiblely,the renal pathologic change relieved significantly, and do not affect the normal kidney.2.For dogs with acute tubular necrosis, human umbilical cord mesenchymal stem cells selectively can survived in the kidney, and have no effect on heart, liver and pancreas.3.The possible mechanism of regeneration was that human umbilical cord mesenchymal stem cells could down-regulate iNOS expression.