Study Of Serum A-fabp, Il-6, Il-17 And Crp In Type 2 Diabetes Mellitus And Obesity

ObjectiveTo investigate the association of serum A-FABP, IL-6, IL-17 and CRP in type 2 diabetes mellitus and obesity.Methods90 patients with type 2 diabetes mellitus (T2DM) were enrolled in the study.45 male,45 female,31～79 age, disease duration 2 months～23 years According to BMI (Body mass index, BMI), they were divided into diabetic non-obese group (BMI<25 kg/m2,48 cases,23 males and 25 females, mean age 49±5.6 years, mean disease duration 9.2±2.7 years) and diabetic obese group (BMI(?)25 kg/m2,42 cases,22 males and 20 females, mean age 50±7.8 years, mean disease duration 10.7±3.4 years). According to macrovascular complicat-ions, they were divided into macrovascular complications group (A group) and non-macrovascular complications group (B group). Obesity group (BMI(?)25 kg/ m2,30 cases,14 males and 16 females, mean age 49±7.6 years), control group (BMI<25 kg/m2,30 cases,16 males and 14 females, mean age 50±4.8 years) diabetic obese group and diabetic non-obese group were matched in age and sex. The levels of serum fasting blood glucose (FBG) and 2-hour postprandial blood glucose (2hPG) were measured by glucose oxidase method; serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), very low density lipoprotein cholesterol (VLDL-C), blood urea nitrogen (BUN), creatinine (Cr), uric acid (UA) and C-reactive protein (CRP) were detected by biochemical method; Glycated hemoglobin (HbAlC) were measured by high performance liquid chromatography (HPLC); fasting insulin (FINS), fasting C peptide (OhC-P),2 hours postprandial insulin (2h-INS), and 2 hour postprandial C peptide in (2hC-P) were measured by magnetic separation enzyme-linked immunosorbent assay; serum adipocyte fatty acid binding protein (A-FABP), interleukin 6 (IL-6) and interleukin 17 (IL-17) by ELISA (enzyme-linked immunosorbent assay). All of the statistical data was processed with SPSS 11.0 software.Results1. Diabetes group compared with the control group and obese group, obesity group compared with the control group,the serum levels of A-FABP, IL-6, CRP, IL-17 and HOMA-IR were higher (P<0.05)2. Diabetic obese group compared with non-obese group, the levels of A FABP, IL-6 and HOMA-IR were increased, while the levels of CRP and IL-17 were not significant.3. A group compared with B group, the levels of A-FABP, IL-6, CRP and IL-17 were elevated (P<0.05).4. The linear correlation and multiple stepwise regression analysis, serum levels of A-FABP is positively correlated with HOMA-IR, CRP, IL-6, significantly positively correlated with BMI, WHR, FBG, TG, HbA1C, and macrovascular complications(P<0.01), negatively correlated with HDL-C (P< 0.05), no correlation with IL-17 (P>0.05).Conclusion1. Serum A-FABP, IL-6, CRP, IL-17 levels and HOMA-IR are increased in diabetes and obesity. The A-FABP, IL-6, CRP, IL-17 are risk factors of obese and insulin resistance.2. A-FABP together with inflammatory cytokines (e.g, IL-6, CRP, IL-17) take part in the development of diabetes and vascular complications.3. Serum A-FABP level is positively correlated with HOMA-IR, CRP, and IL-6 (P<0.05), significantly positively correlated with BMI, WHR, FBG, TG, HbA1C, and macrovascular complications(P<0.01), negatively correlated with HDL-C (P<0.05), no correlation with IL-17 (P>0.05).4. IL-17 as a new inflammatory factor may be a support of study the pathogenesis of diabetes and vascular complications.