Experimental Study Of Electric Acupuncture Combined With Bmscs Transplantation For The Treatment Of Ischemic Cerebral Vascular Disease

Objective:To study the possibilities of electrical acupuncture in promoting the differentiate of bone marrow stromal cells to neuron-like cells in the brain of rats. By electro-acupuncture combined bone marrow stromal stem cells transplantation on cerebral ischemia infection of neurological function to explore the possible mechanism.Methods:BMSCs were purified and amplified by methods of adherence in vitro; Middle Cerebral Artery Occlusion (MCAO) model of rats was copied by fishing thread, and the successful models were randomly divided into control group, electrical acupuncture group, BMSCs group and combination group, adding sham group there are five groups in all.After treatment, we observed the changes of rats'neuron severity scores (NSS), morphology of brain tissue, BDNF expression level in ischemic hemisphere of brain tissue and BMSCs'survival condition.Results:Electrical acupuncture group, BMSCs group and combination group significantly improved the rats'neurological behavior, pyramidal cell structure of ischemic cortex, increased the expression level of BDNF comparing with control group and the combination group has the most obvious approvement; The survival cells which came from transplantation in brain tissue of combination group are more than BMSCs group.Conclusions:After transplating BMSCs can survive and differentiate to electrical acupuncture and BMSCs transplantation in cmbination have synergistic effect.The mechanisms may have relation to improving microenvironment of ischemic brain tissue, promoting the survival of transplanted BMSCs and secret of BDNF'expression level.