Establishment Of Quality Standard And Fingerprint Chromatograph Of Ipomoea Batatas (l.) Lam. Simon No.1

Objective Establishing the quality standard of medicinal materials of Simon 1. In this experiment,10 batches of Simon 1 and 7 kinds of sweet potatoes leaves from Hunan as experiment objects for comparative study, and taking reseach to find the differences between the leaves of Simon 1 and the leaves of the other sweet potatoes by means of morphology, microstructure and thin layer chromatography. Establishing the fingerprint chromatography of medicinal materials of Simon 1.Methods This research was divided into two parts as follow.1. To study the differences between Simon 1 leaves and the other ordinary sweet potatoes leaves from Hunan by morphological and microscopic observation,and research for moisture content, total ash, acid insoluble ash and other items of inspection of Simon 1.2. Accoding to the chemistry ingredient and pharmacological effect of the leaves of Simon 1, we compared with differences of chromatogram conditions and viewing conditions between Simon 1 leaves and the other ordinary sweet potatoes leaves from Hunan by the method of TLC.Developing agent was chloroform-methanol-anhydrous formic acid (8:2:0.2); The injection volume was 2μL; The plate was silica gel G plate; Reagent was 5%aluminum chloride ethanol solution (365nm wavelength UV lamp) and 10%sulfuric acid alcohol solution (sunlight) To establish high performance liquid chromatography method for determinating caffeic acid in medicinal materials Simon 1. Using Phenomenex C18 column (250 mm x 4.6 mm,5μm), with 0.1%HAC-acetonitrile (88:12) as mobile phase; column temperature was 20℃; The detective wavelength was at 325nm,and flow rate was 1 mL#min-1.To analyze 10 batches of medicinal materials Simon 1 by RP-HPLC, and investigated the fingerprint of medicinal materials Simon 1 in different extraction conditions and different chromatographic conditions.Separation was performed on Phenomenex-Cis column (250 mm x 4.6 mm,5um),0.05%phosphoric acid-0.05%phosphoric acid methanol solution as mobile phase with gradient elution; The flow rate was 1 mL·-min-1;The column temperature was 30℃; The detective wavelength was 325nm; The detective time was 110 minutes.Result The significant difference of chromatographic spot between Simon 1 leaves and the other ordinary sweet potatoes leaves from Hunan after eluting with 95%ethanol by polyamide column chromatography.There are differences between Simon 1 leaves and the other ordinary sweet potatoes leaves from Hunan in plant morphology and microscopic features.The content of caffeic acid of medicinal materials of Simon 1 were significantly higher than other sweet potatoes leaves'.Established quality standards of medicinal materials of Simon 1 after investigation of different test items of medicinal materials of Simon 1. Eight common peaks in chromatograms were separated from the 10 batches of medicinal materials of Simon 1 and the common pattern of them was established by HPLC.Conclusion The quality standard and the fingerprint of Simon 1can be used to control the quality availably.