Clinical Significance And Detection Of Mutations On Hepatitis B Virus Pres Region

Objective: analyze the sequence of the preS gene in the HBV-infected patients from ChongQing regional cohort; study the sites, frequencies and types of preS genovariation; explore the features of preS genovariation; invest the correlation between preS genovariation different progression and prognosis.Methods:38 HBV-infected patients from the first hospital affiliated ChongQing Medical University during March, 2009-November, 2009 were recruited into the present study, including 13 cases of asymptomatic carriers (ASC), 19 cases of patients with chronic HBV infection (CHB) and 6 cases of patients with hepatocellular carcinoma (HCC). PCR was employed for the amplification of the HBV preS gene, and PCR product was sequenced, followed by its blast to the sequence of the wild-type EF325674 strain in the GeneBank and the mutated sites were screened out. Results: 5 gene mutations were detected in the T-lymphocyte recognition region of the coding sequence of the preS gene. The frequencies of the codon mutations at 155,165 in CHB groups were significantly higher than in ASC groups, respectively(P<0.05). There were no difference among ASC groups, CHB groups and HCC groups in frequencies of the codon mutations at 35, 39,48. The frequencies of the codon mutations at 35,155 in high- viremia groups were significantly higher than in low- viremia groups, respectively(P<0.01).Conclusions: HBV preS gene mutation plays important role in both the pathogenesis and progression of HBV infection. The codon mutations at 155,165 were more easily result in HBV chronicity especialy. There were little correlation between the codon mutations at 35, 39,48 and the pathogenesis and progression of HBV infection. Objective: To establish the routine detection on preS gene mutation by RDB.Methods:The oligonucleotide probe was designed according to the codon mutations at 155th,165th. The mutated sites were detected by reverse dot blot (RDB).Results: The coincidence between the results from RDB detection and DNA sequencing was 100%.Conclusions: Detection of the preS gene mutation by RDB is a cost-effective, rapid and reliable method, which is suitable for routine detection in hospitals.