| Subject To find much better methods for the management of chronic osteomyelitis,we developed an plastic nano-hydroxyapatite nano-HA/collagen (C)gentamicin (GM)drug delivery system (DDS)(nano-HA / C-GM-DDS) and explored the efficiency of nano-HA/C-GM-DDS in vivo release experiment in rats.Methods:(1)preparation of nano-HA/C-GM-DDS:nano-HA used as the core carrier GM and coated with degradable and biocompatible materials of C, bears the role to load GM.(2) In vivo release experiment:after preparation,nano-HA/C-GM Polymer were implanted in the holes made at quadriceps femoris of 51 experimental rats through operations under anesthesia state. Six rats were selected respectively and quadriceps femoris were obtained on the 1st day, 2nd day, 3rd day, 5th day, 7th day, 9th day, 12th day, 18th day, 24th day after implantation. SPSS 10.0 statistical software package was applied in the drawing of relationship curve between time and drug release concentration of nano-HA/C-GM-DDS after calculating GM concentration of each sample by standard curve. Experiment results were assessed through the inflammatory response of muscle specimens observed under light microscope as well as the foregoing time-concentration curve. Results On the 1st day after operation, the GM concentrations in quadriceps femoris around nano-HA /C-GM-DDS were (130.35±9.25)mg/L,then a decrement tendency could be observed, but on the 24th day the GM concentrations in quadriceps femoris were (5.25±1.01)mg/L, which still exceed the minimal inhibitory concentration(2 mg/L) for the staphylococcus aureus.Conclusion In short, the nano-HA /C-GM-DDS has better slow-release properity in vivo,which can be used as an effective biological material in the management of bone tissue infection. |
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