Ang-1 And Vegf Expression In Early Diabetic Retinopathy In Rats

Objective:To explore the angiopoietin -1 (angiopoietin-1, Ang -1) and vascular endothelial growth factor (vascular endothelial growth factor) in the early expression and significance with the retina of diabetic rats.Method:Make similar mankind a I type diabetes big rat animal model, Take 40 SD male rat divide the group of diabetes 28 and normal matched control group is 12, The group of the diabetes 28 SD male rats intraperitoneal injection the STZ60 mgs.Kg-1, after setting up mold success, distinguish in a week,2 weeks,3 weeks,4 weeks to take the set of the diabetes 7 for each and normal matched control 3 to harvess a rat eyeball, adopt the immunity method examination Ang-1, VEGF in see the retina.Result:Ang-1 in the retina of normal control big rat is no expression in 1-4 weeks, at 1-3 weeks, the diabetes big rat retina sees internal limiting membrane(ILM), inner nuclear layer(INL), ganglion cell layer(GCL);,and spread at the blood vessel cell immunity to respond that the positive reaction, and the positive reaction display palm tree beside yellow, align in the endochylema of Beale's ganglion cells, the Muller cell and juxtavascular cell, the positive expression become up-trend, the retina vessel wall blank increase breadth and retina thickness become thin in 4 weeks the diabetes big rat, but afferent appearance have no obvious change, the Ang-1 positive expression rate lower and have already become a negative expression.The positive expression rate of Ang-1 in the fist week of diabetes big rat is about70.0%,;the second week, the positive expression rate is about 80.0%;the third week, positive expression rate is about 83.3%, the fouth week, the positive expression rate has already change the negative,and the positive expression for about 22.2%. The positive expression rate of Ang-1 in the group of the diabetes 4 weeks,carries on X2 examinations, X2=21.67, P=0.010(P<0.05) and have covariance to learn meaning.VEGF in 1-4 weeks normally big rat matched control retina all is clear the negative dyes reaction, but in 1-4 weeks, diabetes model group retina pathological section is clear to align in the bipolar cell of inner nuclear layer(INL), purpurogenous membrane and spread at vascular endothelial cell(VEC), the M u ller cell staining reaction, appear for the 1-3 weeks high expression up-trend, the 4 weeks, expression has to descend, but still in the high scope inside. The VEGF expresses in the retina of diabetes big rat,the fist week the positive expression rate is about 75.0%, the second week positive expression rate is about 85.9%, the third week positive expression rate is about90.0%, the fouth week positive expression rate is about 73.2%.The internal limiting membrane(ILM) also sees the shallow brown weak positive respond in parts of retina pathological section;the 4 week, the diabetes retina vessel wall blank increase breadth.The group of the diabetes of 4 weeks VEGF positive expression the rate carry on X2 examinations, X2=14.45, P<0.05, Normal matched control and the 1～3w group of the diabetes the positive expression rate of the Ang-1 carrying on covariance to learn analysis have already shown significant difference (P<0.01), but and the 4w the group of the diabetes the positive expression rate of the Ang-1 carrying on covariance to learn analysis have already shown not difference (P>0.05), Normal matched control and the group of the diabetes the positive expression rate of the Ang-1 carrying on co variance to learn analysis have already shown significant difference P<0.05.Conclusion:1,diabetes big rat's retina have already appeared an obvious change for 4 weeks:See the retina thickness become thin, the vessel wall blank increase breadth, didn't see juxtavascular cell.2,the Ang-1, VEGFof diabetes in earlier period big rat see 1-3 weeks in the retina appear a high expression, the 4 week expression descend, and twos appear to separate phenomenon, conjecture along with the extension of the course of illness, vascular permeability(VP) along with increasing, the expression of Ang-1 would less and less, but the VEGF expression will be more and more.3,the Ang-1 be an anti- to permeate factor, VEGF in order to permeate factor, there is important function in the vessel integrity, two after lose the balance then appear blood vessel to permeate, produce factor of stimulate the afferent freshman to release thus. Identify the expression of Ang-1 in the earlier period diabetes retina,it is one of the measures of the treatment to interfere in the diabetes retina early days.