Effects Of Anti-vascular Endothelial Growth Factor Avastin On The Growth Of Cultured Rabbit Retinal Muller Cells

Objective To investigate effects of anti-vascular endothelial growth factor Avastin on the growth of cultured rabbit retinal Muller cells, so as to learn the molecular mechanism of the curation of avastin to diabetic retinopathy(DR) especial the proliferative diabetic retinopathy (PDR).Methods In vitro, rabbit retinal Muller cells were successfully cultured from healthy rabbit by way of tissue adherence. We chose the rabbit retinal Muller cells of P2generation and planted them in six holes cell culture plate for experiment. Then cultured Muller cells were divided into Avastin groups, Avastin+FBS groups, FBS control group and blank control group. The former two groups were respectively treated with five different concentration series of Avastin which was0.05、0.125、0.25、0.5、1.25mg/ml. After treated for24h, the growth of cultured rabbit retinal Muller cells were observed by Flow Cytometry(FCM).Results After tested by Flow Cytometry, we can see that comparing with FBS control group, the growth of cultured retinal Muller cells were significantly restrained in blank control group(P<0.05); comparing with FBS control group, the growth of cultured retinal Muller cells were significantly restrained in Avastin+FBS groups(P<0.05); after treated by the same concentration of Avastin, the Muller cells cultured in Avastin groups were significantly restrained compared with Avastin+FBS groups(P<0.05); intragroup of Avastin groups and Avastin+FBS groups, the growth of cultured retinal Muller cells were significantly restrained and this effect was concentration dependent in a certain range.Conlusion The anti-vascular endothelial growth factor Avastin could restrain the growth of rabbit retinal Muller cells in vitro and this effect was concentration dependent in a certain range which indicates that Avastin may be play a importment role for clinic treatment of PDR.