Experimental Studies Of The Stilbene Light Eyesight Capsule Of Spontaneous Diabetic Rat Retinal Protein Kinase C And Vitreous Vascular Endothelial Growth Factor

Objective: To observe the effect of QiDengMingMu capsule on protein kinase C in retinas and the Vascular endothelial growth factor in vitreous of the experimental animal model of spontaneous diabetic retinopathy rats (Goto-kakizaki rats), and to explore the pathological mechanism of Chinese Medicine on diabetic retinopiathy (DR) .Methods: To evaluate the experimental animal model of spontaneous diabetic retinopathy rats (Goto-kakizaki rat) in morphology. The GK rats with six month course are randomly divided into 6 groups according to the weight and the level of blood glucose:model control group, treated groups with high,middle,low dosage of QiDengMingMu capsule , positive control group of Difral and Doxium, and normal control group, respectively. After given drugs or distilled water for 3 months, the rats were put to death and eyeballs of the rats are taken out. Their vitreous and retinas were studied by means of enzyme linked immunosorbent assay (ELISA) and immunohistochemistry respectively.Results:(1)The experimental animal model of spontaneous diabetic retinopathy rats may represet background stage of diabetic retinopathy in human, especially the experimental animal model of non-insulin dependent diabetic retinopathy. (2)The Chinese medicine QiDengMingMu capsule has the effect of improving the quality of life of the GK rats. (3)The Chinese medicine QiDengMingMu capsule can inhibit the activity of PKC of the GK rats in retinas. (4)The Chinese medicine QiDengMingMu capsule can reduce the level of the Vascular endothelial growth facter of the GK rats in vitreous. (5)The decrease in the blood glucose level of the GK rats is partly owing to the Chinese medicine QiDengMingMu capsule.Conclusion:The experimental animal model of spontaneous diabetic retinopathy rats may represet background stage of diabetic retinopathy in human, especially the experimental animal model of non-insulin dependent diabetic retinopathy. The Chinese medicine QiDengMingMu capsule can the inhibit activity of PKC of the GK rats in retinas and can reduce the level of the Vascular endothelial growth facter of the GK rats in vitreous. So it is a good capillary protective agent to the GK rats'retinal impairment and it can cure the damage of retinas in the GK rats.