Expression Of Wwp1 And Its Correlation With Er, Cerbb-2 And P53 In Breast Invasive Ductal Carcinoma

Breast cancer is one of the most common malignant tumors of female which endanger the health of women seriously. In recent years, the incidence increased obviously and has been in the forefront of malignant tumors of women. Breast cancer is a highly heterogeneous tumor, caused by a multi-factor and multi-step complex process. Breast cancer is the combined effects of the diseases by genetic and environmental factors. There are numerous gene mutations in breast cancer. It is a hot spot of clarification the mechanism to early prevention, early diagnosis and early treatment.The balance of proteins in mammalian cells is tightly controlled by protein synthesis, transcriptional regulation and degradation. Selective degradation of these proteins through the UPS plays an essential role in normal cell growth and differentiation, whereas abnormal accumulation or hyperactive degradation of these regulatory proteins is associated with carcinogenesis. The WW domain containing E3 ubiquitin protein ligase 1(WWP1) belongs to the Nedd4-like homologous to the E6-associated protein C terminus (HECT)-type E3 family. The amplification of the q21 band of chromosome 8(8q21) occurs in a large percentage of breast cancers.WWP1,an HECT domain-containing ubiquitin E3 ligase located in the 8q21 region, frequently amplified and over expressed in breast cancer. Growing evidence suggests that WWP1 negatively regulates the TGF-βtumor suppressor pathway.In order to detect the expression of WWP1 in breast cancer, we performed immunohistochemical staining in formalin-fixed and paraffin-embedded tissue section from 15 cases of normal breast tissue,30 cases of normal ductal hyperplasia,30 cases of ductal carcinoma in situ and 97 cases of breast invasive ductal carcinoma, then to detect WWP1 associatiation with ER, CerbB-2 and p53. Further development of WWP1 as a biomarker and therapeutic target for breast cancer management deserves further investigation.Materials and methods1. We select 15 cases of normal breast tissues,30 cases of normal ductal hyperplasia,30 cases of ductal carcinoma in situ and 97 cases of breast invasive ductal carcinoma from The Third People's Hospital of Zhengzhou during the 2005-2009.All of the cases of ductal carcinoma in situ and invasive ductal carcinoma are aged from 36 to 62 years old and have not been any preoperative chemotherapy or radiotherapy.2. We detect the expression of WWP1 in normal breast tissue, usual ductal hyperplasia, ductal carcinoma in situ and breast invasive ductal carcinoma and the expression of ER, CerbB-2 and P53 in breast invasive ductal carcinoma by SP immunohistochemistry. Then to analysis the relationship of WWP1 with ER, CerbB-2 and P53.3. Statistical analysis:the results are dealed with SPSS13.0, test levelα=0.05.Results1. There is no expression of WWP1 in normal breast tissue and usual ductal hyperplasia. Their rates of positive expression is 0%(0/15) and 0% (0/30).WWP1 is over expressed in breast invasive ductal carcinoma and ductal carcinoma in situ. Their rate of positive expression is 41.2%(40/97) and 36.7% (11/30).2. Correlation analysis showed that:The expression of WWP1 was positively correlated with that of ER (rs=0.204,P<0.05)and P53(rs=0.206,P<0.05).And no obvious correlation with that of CerbB-2 (rs=-0.011, P<0.05)Conclusion1. WWP1 is over expressed in breast invasive ductal carcinoma and ductal carcinoma in situ, which may be related to the development of breast invasive ductal carcinoma.2. The expression of WWP1 was positively correlated with that of ER and P53.WWP1 and ER, P53 may play a synergistic role in the process of breast invasive ductal carcinoma.