The Relationship Between The Pre-s1 Antigen In Patients With Chronic Hepatitis B And Hepatitis B Virus Infection In Peripheral Blood Monouclear Cells And Immune Function

Objective Through regarding chronic hepatitis B (chronic viral hepatitis B, CHB) patients as the research object, detect and analyze the relevance between the serum pre-S1 (preliminary-s1, Pre-S1) antigen, serum HBV-DNA (hepatitis B vivas-deoxyribonudeic acid, HBV-DNA) and peripheral mononuclear cells (peripheral blood monouclear cells, PBMC) in the HBV-DNA,in order to find the indicators which are helpful to judge the extent of hepatitis B virus (hepatitis B rivas, HBV) replication in immune cells,observing the extent of immune damage to evaluate the clinical application value of the Pre-S1 antigen in the immune response to HBV replication and the cause of cell immune injury by further detecting the number of CD4+, CD8+T subsets. Methods This was in March 2008 to August, in the Ningxia Medical University Hospital infection out-patient and inpatient treatment of 108 patients with CHB, and out-patient physical examination 30 patients with healthy controls, serum and PBMC isolated reserve. Enzyme-linked immunosorbent assay (enzyme-linked immunsorben tassay, ELISA) detected 108 cases of CHB patients with Pre-S1 antigen, using fluorescence quantitative polymerase chain reaction (fluorescent quqntity polymerase chain reaction, FQ-PCR) detected in the serum and PBMC The HBV-DNA, while using flow cytometry (f1ow cytometry, FCM) to detect all patients and healthy control subjects were T cell subsets in the circumstances, to do statistical analysis. Results 1.Pre-S1 antigen in HBV replication reaction diagnostic sensitivity 89.7%, 91.7% accuracy is better than hepatitis B e antigen (hepatitis B e antigen, HBeAg). 2.Pre-S1 antigen positive rate and the serum and PBMC of HBV-DNA are an association (X²_serum = 75.19, X²_PBMC = 47.79, P <0.01, r_serum = 0.96, r_PBMC = 0.98), and HBV-DNA content with increases. 3.CHB patient group than in normal healthy control group, CD3+ (T = 3.25, P = 0.024), CD4+ (T = 3.05, P = 0.038) T cell percentage and CD4+ / CD8+ ratio (T = 3.94, P = 0.017) significantly reduced, CD8+ T cell percentage was significantly higher than the healthy group (T = -3.7, P = 0.021). 4.Pre-S1 antigen-positive group than in negative CD3+ (T = 2.7, P = 0.04), CD4+ (T =- 2.4, P = 0.02) T cell percentages, CD4+ / CD8+ ratio (T = 3.4, P = 0.02) decreased, CD8+ T cell percentage (T = 2.9, P = 0.04) were significantly increased. 5.PBMC in HBV-DNA positive group and HBV-DNA negative group, CD4+ T cell percentage (T =- 2.5, P = 0.04), CD4/CD8 ratio were decreased (T = 3.12, P = 0.03), CD8+ T cell percentage was significantly higher (T = 3.8, P = 0.01), CD3+ T cells (T = 4.5, P = 0.07) no significant difference. 6.PBMC in the amount of HBV-DNA and CD3+, CD4+ were negatively correlated (r = -0.63, -0.51) (P <0.01), HBV-DNA of the load and CD8 + positive correlation (r = 0.61 ) (P <0.01).Conclusion 1.Pre-S1 antigen reaction test of HBV replication performance than HBeAg. 2. Serum Pre-S1 antigen and serum HBV-DNA and PBMC in the presence of HBV-DNA and replication are closely related, Pre-S1 reflects HBV replication in immune cells, the degree of immune response to injury situation. 3. The human body occurs in patients with chronic hepatitis B immune T cell disorder, HBV infection after PBMC with HBV-DNA replication in PBMC increased to further increase the T cell dysfunction, affecting their immune function. Therefore, HBV can infect PBMC, Pre-S1 antigen may reflect the damage of cellular immunity in patients with CHB can be used as diagnosis of hepatitis B, HBV replication and infection of PBMC response to a sensitive indicator of chronic hepatitis B patients with Pre-S1 antigen in serum and PBMC Combined detection of HBV-DNA can determine the condition of patients with hepatitis B, clinical treatment and provide a reliable basis for evaluation.