| ObjectiveTo a certain unit of 174 male to carry out research to explore the different drinking, started drinking in different age, different time drinking, drinking frequency and different drinking types of liver function on the body, also observed in the oxidative stress factor in serum malondialdehyde (MDA), metallothionein (MT) content changes, specifically on the degree of liver damage alcohol effects, while clinical interventions and health education to provide information support for the development of health services and provide evidence.Methods1 Data collection:During march to septemberr in 2009, in North China Coal Medical College Hospital-line health check of a unit of 174 male for the study, carried out a unified self-made questionnaire survey. Inclusion criteria: (1) age older than 18 years of age (2) voluntary participation in this survey. Exclusion criteria: (1) age older than 80 years of age (2) alcohol or other important information is not complete information is not complete by (3) alcohol, hepatotropic virus infection, drugs and toxic liver damage, diabetes, hepatitis, cirrhosis and cancer of the critical illness. Development of the questionnaire, after repeated inquiries by the unified pre-training survey professionals, calibration equipment for physical examination, by the strict training of qualified medical and health technicians in the field height and weight measurements. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), r-glutamyl peptidase (GGT) and other related ancillary test results by the North China Coal Medical College Hospital medical centers, all biochemical tests by experienced, skilled professional doctors in the same laboratory operation is complete.2 Blood sampling: After informed consent, physical examination early in the morning in taking more than 12 h fasting venous blood samples were 5 ml, heparin and centrifuged, and serum extracted, placed in cryogenic refrigerator tested. 7:00-7:30 in the morning blood at all times.3 Laboratory tests: serum oxidative stress factor MDA (MDA) was determined by thiobarbituric acid (Thibabituric Acid TBA) method, free radical scavenger metallothionein (MT) was determined by double antibody sandwich ELISA method All detection experiments were professional help operators complete kit purchased from Nanjing Jiancheng Biological Engineering Institute4 Data Processing: Investigation of the general situation, including age, ethnicity, educational level, occupation, marital status, income, exercise status, smoking history, body mass index (BMI), etc.; past history included coronary heart disease, hypertension, congenital heart disease, peripheral arterial disease, cerebrovascular disease, diabetes, hepatotropic virus infection, drugs and toxic liver damage, hepatitis, liver cirrhosis and cancer of the critical disease; alcohol consumption, including the beginning of drinking age, drinking time, drinking wine type, the average per-alcohol consumption, average weekly frequency of drinking, alcoholic beverages according to alcohol consumption = (ml)×alcohol content (%)×0.8 estimated average daily alcohol consumption; on the strict control of data input data quality.5 Statistical Methods: All information on building a database using Excel 2003, SPSS 13.0 statistical software to process data.Measurement data to S that count data or the form with frequency than that. Stratified analysis controlling for confounding factors, measurement data between multiple samples were compared by a number of analysis of variance and q test, count data compared byχ2 test.Results1A total of 174 selected subjects, all male, of which 138 were alcohol, 36 people never to drink alcohol was 79.31%. In addition to education and physical activity, alcohol group and does not constitute a drinking group with statistical significance, (P <0.05), other differences between the two groups were not statistically significant (P> 0.05), show that the two groups good comparability between2 The serum alcohol ALT, AST, GGT levels higher than non-drinking group.Different alcohol consumption, different start drinking age, drinking times and different drinking frequency, among groups of different alcohol types of serum ALT, AST, GGT levels were significant differences . With the increase in alcohol consumption, drinking age in advance, prolonged drinking, drinking frequency accelerated the trend of the three indicators have increased, indicating the degree of liver injury more and more different types of alcohol to the extent of liver injury the lightest wine, beer second, liquor, beer, liquor mixed the most serious. 3Alcohol intake on serum MDA, MT content3. Different alcohol consumption, different start drinking age, drinking times and different drinking frequency, among groups of different alcohol types of serum MDA, MT content of the differences were statistically significant. With the increase in alcohol consumption, drinking age in advance, prolonged drinking, drinking frequency speed, MDA levels are rising trend, MT levels have decreased, indicating more intense oxidative stress, different drink wine types the extent of oxidative stress caused by the lightest wine, beer second, liquor, beer, liquor mixed the worst.Conclusions1 Alcohol consumption increased, began drinking age in advance, prolonged drinking, drinking frequency speed, high consumption of alcoholic liquor so potent it can increase the bad drinking habits are drinking on the liver functional injury.2 Drinking can cause the body's oxidative stress response and increased with alcohol consumption, prolonged drinking, drinking frequency speed, gradually increased oxidative stress, delay the commencement of drinking age, drinking red wine and other alcohol is relatively little alcohol can reduce the degree of oxidative stress, thus reducing the liver damage. |
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