Studies On The Processing Procedures Of Huperzia Serrata And Hupa In-situ Forming Gel

Huperzia Serrata(Thunb.)Trev.,is a common Chinese traditional medicine mostly used in traditional Chinese prescriptions.Huperzia Serrata is capable of expelling toxin and heat by cooling.In 1980s,it is extracted a kind of super activated alkaloid named Huperzine A from the plant of Huperzia Serrata,which was used medicine of curing AD.Because Huperzia Serrata(Thunb.)Trev.resourceses distribute like star to spread,the resources renewal period is longer,which can be provided to mine of the natural resources is small,for the sake to better rightness existing resourceses of reasonable of exploitation,this text is used the processing system method of the vinegar system,wine system to withdraw the content of alkaloid of Hup A in thing by exaltation.Study the result enunciation,thousand layer towers of the wine system,vinegar system withdraw alkaloid of Hup A content in thing to distinguish to 0.016%,0.020%,all compare to living an article to withdraw alkaloid of Hup A content 0.0095%in the thing to raise 100%or so,so put forward new means for thousand reasonable exploitation of the layer towers.Make use of the modem color table means the separation in thousand total alkalis in the layer tower to get 2 compounds from the vinegar system,and learn a method to authenticate of Hup A and Hup A through the chemistry and spectrum respectively.(the Huperzine B,the Hup B).Measurese Huperzia Serrata(Thunb.)Trev cannon system crafts and withdraw the content of Hup A in the craft a measurement a method through an establishment,make use of a single factor investigation and is hand over the design experiment,end build up thousand layer tower best vinegar system crafts with superior withdraw the condition of the craft.According to theory of processing Chinese herbel medicine,we processed the dried Huperzia Serrata(Thunb.)Trey.with rice vinegar.The systematic investigation on chemical constituents led to the isolation of two compounds.On the basis of chemical evidences and spectral analysis,the structures of the 2 compounds were elucidated,and they are Huperzine A and Huperzine B.In vitro and in vivo analysis methods were established and the basic physiochemical propertied of Huperzine A.It was indicated that the in vitro/in vivo analysis method was reliable and simple;the solubility of Huperzine A was at least 1 mg/ml in different aqueous solutions;the partition coefficient in n-octanol/water system at 25℃was 10.02;Huperzine A was very stable in PBS 7.4.Huperzine A loaded copolymer were successfully prepared from PLGA-PEG-PLGA. The effect of formula and preparative parameters on in vitro drug release behavior was studied in detail,based on which the best efficiency formulation and preparation conditions was determined.The reproducibility and stability of the best formulation were good.The in vivo performance of the PLGA-PEG-PLGA copolymer was studied.The pharmacokinetic study in rats showed that the PLGA-PEG-PLGA copolymer could sustain The result express:Hup A can inject slowly T_maxwas a 3 h;C_maxwas(112.96±s 10.64)ng·ml^-1;AUC_0â†'Twas(2331.304±364.90)ng·h·ml^-1;AUC_{0â†'∞}was(3016.224±400.57) ng·h·ml^-1;t_1/2was(39.420±2.41)h.Release time as 3～slowly for 4 days in the body,sex pass inside the body is good.