Expression And Significance Of P-mtor And P-p70s6k In Benign And Malignant Endometrical Diseases

Background and objective:Endometrial carcinoma is also called corpus carcinoma,it's a malignant disease which derives from normal epithelial,most of it derives from endomembrane gland.so it was called endometrial adenocarcinoma or endometioid adenocarcinoma. Endometrial carcinoma is one of the three most common malignant tumors in genital duct,to occupy 20-30%in malignant tumors in female genital system.Though it has a better prognosis,the incidence of endometrial carcinoma has a tendency of increase in the world.In some Euro-American countries,endometria]carcinoma is the most common gynecological tumor of genital duct.the advanced stage endometrial carcinoma has a worse prognosis,moreover it's clinic therapeutic methods is limited. At present,its molecular mechanism has not exactly definite.Therefore,it is very important to explore its molecular mechanism for endometrial carcinoma's diagnosis, therapeutics and assess its prognosis. Recent findings reveal that mammalian target of rapamycin(mTOR) is a central regulator of cell growth,P70S6K is one of mTOR's downstream effector,is a key factor for protein synthesis in a variety of cell types.Thus,mTOR/P70S6K signal transduction may play a vital regulation in the genesis and development of endometrial carcinoma.At present,the research about this aspect is seldom.In order to investigate the possible mechanism of p-mTOR,p-P70S6K in the genesis and development of endometrial carcinoma,provide the theory basis for clinical diagnosis and treatment.The expressions of p-mTOR and p-P70S6K in tissues from endometrial adenocarcinoma,simple endometrial hyperplasia, endometrial atypical hyperplasia,and normal endometrium were detected by using S-P immunohistochemistry stain.Materials and methods1.The tissues:the 49 cases of endometrial adenocarcinom,11 cases of simple endometrial hyperplasia,11 cases of endometrial atypical hyperplasia and 10 cases of normal endometrium paraffin-embeded tissues came from the pathological department of the Third Affiliated Hospital of Zheng Zhou University and the People's Hospital of Henan province,date from August 2007 to June 2008.According to the operational and pathological stage:carcinoma in situ:12,stageⅠ:15,stageⅡ: 12,stageⅢ～Ⅳ:10.There are 6 cases have the lymphatic metastasis.All the patients had not accepted the chemotherapy,radiotherapy and hormone therapy.The age of patients ranged from 26 to 86.2.Methods:The expression of p-mTOR and p-P70S6K were detected by immunohistochemistry SP method.3.Statistical analysis:All the date was computed by using SPSS13.0 statistics software.The mean of each group was compared by one-way ANOVA.The difference of average in two groups was compared with t-test.The correlation of p-mTOR and p-P70S6K was analysised by pearson correlation.The criterion for statistical significance was a=0.05. Results:1.p-mTOR was located mainly in cellular endochylema.Each group's mean OD: proliferative phase endometrium:46.59±3.39,simple endometrial hyperplasia: 53.33±4.07,endometrial atypical hyperplasia60.43±2.95,carcinoma in situ: 68.14±4.28,stageⅠ:74.95±5.32,stageⅡ:85.37±4.24,stageⅢ～Ⅳ:89.47±4.37. lymphatic metastasis:91.67±2.97,nonlymphatic metastasis:76.67±8.31.The results of one-way ANOVA is between each two group,there was a significance statistical difference,P＜0.05.lymphatic metastasis' group and nonlymphatic metastais' group were calculated by t-test,there was a significance statistical difference when p＜0.05.2.p-P70S6K was located mainly in cellular endochylema and nucleus.Each group's mean OD:proliferative phase endometrium 53.34±2.43,simple endometrial hyperplasia 71.24±3.38,endometrial atypical hyperplasia 77.38±3.54,carcinoma in situ:81.66±3.68,stageⅠ:89.52±6.03,stageⅡ:95.95±5.32,stageⅢ～Ⅳ: 100.92±6.00,lymphatic metastasis:102.03±5.82,non lymphatic metastasis: 89.73±7.85.The results of one-way ANOVA is between each two group,there was a significance statistical difference,P＜0.05.lymphatic metastasis' group and nonlymphatic metastais' group were calculated by t-test,there was a significance statistical difference when p＜0.05.3.Between p-mTOR and p-P70S6K was analysised by pearson correlation,and r=0.947(p＜0.01),indicated that they are linear positive correlation.Conclusions1.The expression of p-mTOR and p-P70S6K is direct ratio to endometrium's hyperplasia degree,indicated that mTOR/P70S6K signal transduction may be participate in ccurrence of endometrial adenocarcinoma.2.The expression of p-mTOR and p-P70S6K in lymphatic metastasis' group is higer than nonlymphatic metastais' group,indicated that mTOR /P70S6K signal transduction maybe play a important effect in endometrial adenocarcinoma's infiltration and metastasis.3.There is a linear positive correlation between p-mTOR and p-P70S6K. indicated that the blocking agents of mTOR maybe use to cure endometrial adenocarcinoma.