| Background and ObjectiveUrokinase was a fiber-activator that can activate blood clots in the pro fibrinolysis, with lower blood viscosity and fibrinogen to prevent platelet aggregation, to dissolve blood clots broken, and other functions. The fresh thrombosis effective. It was reported in the literature, urokinase eyes injection can be treated hyphema and vitreous hemorrhage, and other hemorrhagic disease. UK will be injected into the vitreous cavity of the toxicity of how to report less. Retina of the physical and chemical effects of drug resistance is weak, so vitreous cavity injection drugs can lead to excessive concentration of retinal toxicity. As the organization has a fine eye and the vulnerability of the special nature of urokinase in the application of the treatment of eye diseases before their eyes, the security is necessary to first evaluate .This study is by looking at different concentrations of urokinase rabbit vitreous cavity after injection study of the retina, parallel electroretinogram (ERG) to assess their function of the retina, with the aim of understanding the concentration of the increase in the retina of urokinase The toxic effect of the changes of its best treatment concentration. To explore the application of local administration and the concentration of urokinase way to provide a basis. Central retinal artery occlusion (CRAO) was serious damage to the eye of visual function. CRAO can cause irreversible vision loss. Embolic obstruction was the main reason. Currently CRAO no special treatment, systemic application of thrombolytic drugs, in the eyes difficult to achieve an effective local concentration, increasing doses will cause systemic important organs, such as serious bleeding complications. In recent years the internal carotid artery or ophthalmic artery intervention urokinase thrombolytic drugs to treat CRAO, but a cerebral hemorrhage and the risk of developing complications such as cerebral infarction.Materials and methods1. Rabbit will be 40 healthy adult rabbits were randomly divided into four groups (the normal group, the vitreous cavity injection 2000 U, 3000U and 5000 U urokinase), each of the 10 rabbit eyes, his right eye for eye tests, his left eye for eye control, Control eyes were injected 0.1 ml PBS. ERG 15 days after the trip to assess their function of the retina, injecting urokinase six months after the animals were killed eye removal, light and transmission electron microscopy observation of the retina pathological changes.2. Rabbit injection method amide compound eye drops, scattered and care for the pupil, ketamine hydrochloride injection by 1.5 ml / kg dose intramuscular narcotic animals. Temporal corneoscleral margin at the top after the 3-4 mm needle, needle slant upward, first in the sclera creeping within 1 to 2 mm, the needle to the direction of the eyeball centre, needle depth of 5 mm, 0.1 ml of urokinase injection Slowly injected into the vitreous cavity rabbit.Results1. ERG change: ERG of a volatility: the experimental group and control group with no statistical difference (P> 0.05).ERG amplitude of the wave- b: 2000U and 3000 U groups: the experimental group and control group showed no significant change, the difference was not significant difference (P> 0.05). 5000U group and the control group, the differences were significant (P <0.05). 2. Optical microscope observation: the group of eyes and control eye retina not unusual. Retinal tissue structural integrity, RGC neatly arranged, in the plexiform layer were more evenly; core and outer layer of cells with a neat; cone rod arranged neatly.3. Electron microscopy Perspective: 3000U group, rabbit retinal pigment epithelial slender reduction processes, film festival, set integration, the gap between the section expanded slightly. Vitreous cavity injection urokinase 5000 U group, the slender black-epithelial cells decrease in the number of processes, sparse, the decrease in the number of sections, with disorder, most film-integration disappear. Mitochondrial membrane fusion of the ridge and disappear and, within a film festival double-integration and missing.Conclusion1. Rabbit vitreous cavity injection 2000u urokinase group, no retinal pathological changes.2. Rabbit vitreous cavity injection 2000u urokinase group 3000U and 5000 U can lead to retinal tissue and festivals and festivals of injury. With the increased concentration, the greater the toxicity of the retina.3 . Rabbit vitreous cavity injection 5000u urokinase group, ERG-b volatility: The eyes than the control eyes b- wave decreased significantly . |
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