Effect Of Ems1-sirna On The Growth In Mgc803 Cell Line

Objective EMS1 gene is a related gene of gastric cancer . MGC803 cell line was selected as a target with positive expression of EMS1 gene and observed the proliferation effect after EMS1 gene expression inhibited by siRNA.Methods pSilencer3.1-EMS1-siRNA expression vector targeting EMS1 gene was constructed and transfected into MGC803 cell line by cationic liposome.The stable pSilencer3.1-EMS1-siRNA/MGC803 cell line was obtained by G418 selection.The levels of the Cortactin protein were detected by western blotting.Cell growth curves and cell cycle distribution were detected in MGC803 cell line after EMS1 gene expression down-regulated.Results The results of DNA sequencing demonstrate that the pSilencer3.1-EMS1-siRNA/MGC803 expression vector was successfully constructed.After pSilencer3.1-EMS1-siRNA and pSilencer3.1 transfected into MGC803 cell,selected with G418 and identified by western blotting,the stable pSilencer3.1-EMS1-siRNA/MGC803 cell line of EMS1 gene down-regulated was established successfully.The growth capacity and FCM analysis of pSilencer3.1-EMS1-siRNA/MGC803 cell not changed after EMS1 gene down-regulated,compared with the controls(p>0.05).Conclusions 1 The expression vector of pSilencer3.1-EMS1-siRNA was constructed successfully.2 The pSilencer3.1-EMS1-siRNA/MGC803 cell line of EMS1 gene expression down-regulated was established successfully.3 The growth of the MGC803 cell line not changed after EMS1 gene expression down-regulated in the pSilencer3.1-EMS1-siRNA/MGC803 cell line.